Development of a Long-Lived Luminescence Probe for Visualizing β-Galactosidase in Ovarian Carcinoma Cells

Wanhe Wang, Kasipandi Vellaisamy, Guodong Li, Chun Wu, Chung Nga Ko, Chung Hang Leung*, Edmond Dik Lung MA

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

75 Citations (Scopus)

Abstract

β-Galactosidase (β-gal) is an important biomarker for ovarian cancers. In this work, we designed and synthesized a novel iridium(III)-based probe 1 for discriminating ovarian carcinoma cell lines from normal cell lines. The probe could detect β-gal even in the presence of a highly autofluorescent background. The probe also showed a good linear response to β-gal between 0 and 30 U/mL, with a detection limit of 0.51 U/mL. Importantly, complex 1 could selectively "light up" ovarian carcinoma cells, while exhibiting negligible luminescence in normal cells. Overall, complex 1 could be potentially used as a useful probe for detecting β-gal expression in the context of ovarian cancer diagnostics.

Original languageEnglish
Pages (from-to)11679-11684
Number of pages6
JournalAnalytical Chemistry
Volume89
Issue number21
DOIs
Publication statusPublished - 7 Nov 2017

Scopus Subject Areas

  • Analytical Chemistry

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