TY - JOUR
T1 - Development of a Long-Lived Luminescence Probe for Visualizing β-Galactosidase in Ovarian Carcinoma Cells
AU - Wang, Wanhe
AU - Vellaisamy, Kasipandi
AU - Li, Guodong
AU - Wu, Chun
AU - Ko, Chung Nga
AU - Leung, Chung Hang
AU - Ma, Dik Lung
N1 - Funding Information:
We would like to thank Dr. Wen-An Qiang (Department of Obstetrics and Gynecology, Feinberg School of Medicine at Northwestern University, Chicago) for kindly providing SKOV3 and OVCAR3 cell lines. This work is supported by Hong Kong Baptist University (FRG2/16-17/007), the Health and Medical Research Fund (HMRF/14130522 and 14150561), the Research Grants Council (HKBU/12301115 and HKBU/201913), the National Natural Science Foundation of China (21575121 and 21628502), the Guangdong Province Natural Science Foundation (2015A030313816), the Hong Kong Baptist University Century Club Sponsorship Scheme 2016, the Interdisciplinary Research Matching Scheme (RC-IRMS/15-16/03), Innovation and Technology Fund (ITS/260/16FX), Matching Proof of Concept Fund (MPCF-001-2017/18), the Science and Technology Development Fund, Macao SAR (098/2014/A2), and the University of Macau (MYRG2015-00137-ICMS-QRCM, MYRG2016-00151-ICMS-QRCM and MRG044/LCH/2015/ICMS).
Publisher copyright:
© 2017 American Chemical Society
PY - 2017/11/7
Y1 - 2017/11/7
N2 - β-Galactosidase (β-gal) is an important biomarker for ovarian cancers. In this work, we designed and synthesized a novel iridium(III)-based probe 1 for discriminating ovarian carcinoma cell lines from normal cell lines. The probe could detect β-gal even in the presence of a highly autofluorescent background. The probe also showed a good linear response to β-gal between 0 and 30 U/mL, with a detection limit of 0.51 U/mL. Importantly, complex 1 could selectively "light up" ovarian carcinoma cells, while exhibiting negligible luminescence in normal cells. Overall, complex 1 could be potentially used as a useful probe for detecting β-gal expression in the context of ovarian cancer diagnostics.
AB - β-Galactosidase (β-gal) is an important biomarker for ovarian cancers. In this work, we designed and synthesized a novel iridium(III)-based probe 1 for discriminating ovarian carcinoma cell lines from normal cell lines. The probe could detect β-gal even in the presence of a highly autofluorescent background. The probe also showed a good linear response to β-gal between 0 and 30 U/mL, with a detection limit of 0.51 U/mL. Importantly, complex 1 could selectively "light up" ovarian carcinoma cells, while exhibiting negligible luminescence in normal cells. Overall, complex 1 could be potentially used as a useful probe for detecting β-gal expression in the context of ovarian cancer diagnostics.
UR - http://www.scopus.com/inward/record.url?scp=85044618649&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.7b03114
DO - 10.1021/acs.analchem.7b03114
M3 - Journal article
C2 - 28969424
AN - SCOPUS:85044618649
SN - 0003-2700
VL - 89
SP - 11679
EP - 11684
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 21
ER -
