Development of a LC-MS/MS method for the determination of antrodin B and antrodin C from Antrodia camphorata extract in rat plasma for pharmacokinetic study

Yongli Liu, Xin Di*, Xingchao Liu, Wenjin Shen, Kelvin S Y LEUNG

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

A selective and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of antrodin B and antrodin C in rat plasma. Both target compounds, together with the internal standard (diazepam), were extracted from rat plasma samples by liquid-liquid extraction with ethyl acetate. Chromatographic separation was carried out on an Agilent XDB-C8 column with an isocratic mobile phase consisting of acetonitrile and water (70:30, V/V) at a flow rate of 0.5mL/min. The mass spectrometric detection was performed by selected reaction monitoring (SRM) mode via atmospheric pressure chemical ionization (APCI) source operating in positive ionization mode. The assay exhibited a linear dynamic range of 47.6-4760ng/mL for antrodin B and 56.6-5660ng/mL for antrodin C. The intra- and inter-day precision was less than 5.3% and the accuracy was less than 2.7% for both analytes. The validated method has been applied to the pharmacokinetic study of antrodin B and antrodin C in rats following oral administration of Antrodia camphorata extract.

Original languageEnglish
Pages (from-to)781-784
Number of pages4
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume53
Issue number3
DOIs
Publication statusPublished - Nov 2010

Scopus Subject Areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

User-Defined Keywords

  • Antrodia camphorata
  • Antrodin B
  • Antrodin C
  • LC-MS/MS
  • Pharmacokinetics

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