Development and analytical application of an uric acid biosensor using an uricase-immobilized eggshell membrane

Yan Zhang, Guangming Wen, Yehong Zhou, Shaomin Shuang*, Chuan Dong, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

128 Citations (Scopus)

Abstract

An uric acid biosensor fabricated from a uricase-immobilized eggshell membrane and an oxygen electrode was presented. The detection schemes involve the enzymatic reactions of the uricase leading to the depletion of dissolved oxygen level upon exposure to uric acid solution. The decrease in oxygen level was monitored and related to the uric acid concentration. The scanning electron micrographs show the microstructure of the eggshell membrane within which the uricase is successfully immobilized. The effects of enzyme loading, pH, temperature, and phosphate buffer concentration on the response of the biosensor were investigated in detail. The uric acid biosensor has a linear response range of 4.0-640 μM with a detection limit of 2.0 μM (S/N = 3). The response time was less than 100 s. The biosensor exhibited good repeatable response to a 0.10 mM uric acid solution with a relative standard deviation of 3.1% (n = 7). The reproducibility of fabrication of the biosensors using four different membranes was good with a R.S.D. of 3.2%. The biosensor showed extremely good stability with a shelf-life of at least 3 months. Some common potential interferents in samples such as glucose, urea, ascorbic acid, lactic acid, glycine, dl-α-alanine, dl-cysteine, KCl, NaCl, CaCl2, MgSO4, and NH4Cl showed no interferences on the response of the uric acid biosensor. The biosensor was successfully applied to determine the uric acid level in some human serum and urine samples, and the results agreed well with those obtained by a commercial colorimetric assay kit.

Original languageEnglish
Pages (from-to)1791-1797
Number of pages7
JournalBiosensors and Bioelectronics
Volume22
Issue number8
DOIs
Publication statusPublished - 15 Mar 2007

Scopus Subject Areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

User-Defined Keywords

  • Eggshell membrane
  • Uric acid biosensor
  • Uricase

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