Development and analytical application of a glucose biosensor based on glucose oxidase/O-(2-hydroxyl)propyl-3-trimethylammonium chitosan chloride nanoparticle-immobilized onion inner epidermis

Fei Wang, Jun Yao*, Mohammad Russel, Huilun Chen, Ke Chen, Yong Zhou, Brunello Ceccanti, Gyula Zaray, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

A glucose biosensor comprising a glucose oxidase/O-(2-hydroxyl)propyl-3-trimethylammonium chitosan chloride nanoparticle (O-HTCC NP)-immobilized onion inner membrane and a dissolved oxygen (O2) sensor has been successfully developed. The detection scheme is based on the depletion of dissolved O2 content upon exposure to glucose. The decrease in O2 level was monitored and related to the glucose concentration. The biosensor shows linear response to glucose from 0.0 to 0.60mM with a detection limit of 50μM (S/N=3). The effect of O-HTCC NP and enzyme loading, pH, temperature, and phosphate buffer concentration on the sensitivity of the biosensor was studied in detail. The biosensor exhibits fast response time (70s), good repeatability (3.2%, n=10) and storage stability (90% of initial sensitivity after 3-week storage). Common interferents including acetic acid, lactic acid, propionic acid, butyric acid, folic acid, methanol, glycine, dl-α-alanine and dl-cysteine do not cause significant interferences on the biosensor. The proposed biosensor method was successfully applied to determine the glucose content in real samples such as orange juice, red wine and tea drink and the results were comparable to that obtained from a spectrophotometric method. The glucose recovery test demonstrates that the proposed glucose biosensor offers an excellent, accurate and precise method for the determination of glucose in real samples.

Original languageEnglish
Pages (from-to)2238-2243
Number of pages6
JournalBiosensors and Bioelectronics
Volume25
Issue number10
DOIs
Publication statusPublished - Jun 2010

Scopus Subject Areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

User-Defined Keywords

  • Enzyme immobilization
  • Glucose oxidase
  • O-HTCC nanoparticle
  • Onion membrane

Fingerprint

Dive into the research topics of 'Development and analytical application of a glucose biosensor based on glucose oxidase/O-(2-hydroxyl)propyl-3-trimethylammonium chitosan chloride nanoparticle-immobilized onion inner epidermis'. Together they form a unique fingerprint.

Cite this