Determination of two intact glucosinolates in vegetables and Chinese herbs

Zongwei CAI; Ching Yan Cheung; Wai Tang Ma; Wai Man Au; Xiang You Zhang; Albert W M LEE

doi:10.1007/s00216-003-2335-x

Determination of two intact glucosinolates in vegetables and Chinese herbs

Zongwei CAI^*, Ching Yan Cheung, Wai Tang Ma, Wai Man Au, Xiang You Zhang, Albert W M LEE

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

9 Citations (Scopus)

Abstract

A reversed-phase HPLC method was developed for analyzing sinigrin and gluconasturtiin in six vegetable and two Chinese herb samples. A gradient program and mobile phases using methanol and 0.05% trifluoroacetic acid containing 20 mM ammonium acetate allowed sufficient retention and separation of the glucosinolates in the sample extracts. Quadrupole time-of-flight tandem mass spectrometry in negative ion electrospray ionization was used to analyze the fractions collected from the HPLC elution to confirm the identification of the glucosinolates. The levels of sinigrin and gluconasturtiin in the vegetables and Chinese herbs were determined by using an external calibration method. Concentrations of gluconasturtiin in the Chinese herbs were more than 15 times higher than those of sinigrin. Detection limits were 18 nmol g^-1 for sinigrin and 4nmol g^-1 for gluconasturtiin when 50 g of fresh vegetable was analyzed.

Original language	English
Pages (from-to)	827-833
Number of pages	7
Journal	Analytical and Bioanalytical Chemistry
Volume	378
Issue number	3
DOIs	https://doi.org/10.1007/s00216-003-2335-x
Publication status	Published - Feb 2004

Scopus Subject Areas

Analytical Chemistry
Biochemistry

User-Defined Keywords

ESI-QTOF-MS
Gluconasturtiin
Glucosinolates
HPLC
Sinigrin

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10.1007/s00216-003-2335-x

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title = "Determination of two intact glucosinolates in vegetables and Chinese herbs",

abstract = "A reversed-phase HPLC method was developed for analyzing sinigrin and gluconasturtiin in six vegetable and two Chinese herb samples. A gradient program and mobile phases using methanol and 0.05% trifluoroacetic acid containing 20 mM ammonium acetate allowed sufficient retention and separation of the glucosinolates in the sample extracts. Quadrupole time-of-flight tandem mass spectrometry in negative ion electrospray ionization was used to analyze the fractions collected from the HPLC elution to confirm the identification of the glucosinolates. The levels of sinigrin and gluconasturtiin in the vegetables and Chinese herbs were determined by using an external calibration method. Concentrations of gluconasturtiin in the Chinese herbs were more than 15 times higher than those of sinigrin. Detection limits were 18 nmol g-1 for sinigrin and 4nmol g-1 for gluconasturtiin when 50 g of fresh vegetable was analyzed.",

keywords = "ESI-QTOF-MS, Gluconasturtiin, Glucosinolates, HPLC, Sinigrin",

author = "Zongwei CAI and Cheung, {Ching Yan} and Ma, {Wai Tang} and Au, {Wai Man} and Zhang, {Xiang You} and LEE, {Albert W M}",

note = "Funding Information: Acknowledgements This research was supported by Faculty Research Grants (FRG/00–01/II-56) and (FRG/01–02/I-19) from Hong Kong Baptist University and the Equipment Grant-Central Allocation of the Research Grants Council, University Grants Committee of Hong Kong (HKBU-1/00C).",

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doi = "10.1007/s00216-003-2335-x",

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AU - CAI, Zongwei

AU - Cheung, Ching Yan

AU - Ma, Wai Tang

AU - Au, Wai Man

AU - Zhang, Xiang You

AU - LEE, Albert W M

N1 - Funding Information: Acknowledgements This research was supported by Faculty Research Grants (FRG/00–01/II-56) and (FRG/01–02/I-19) from Hong Kong Baptist University and the Equipment Grant-Central Allocation of the Research Grants Council, University Grants Committee of Hong Kong (HKBU-1/00C).

PY - 2004/2

Y1 - 2004/2

N2 - A reversed-phase HPLC method was developed for analyzing sinigrin and gluconasturtiin in six vegetable and two Chinese herb samples. A gradient program and mobile phases using methanol and 0.05% trifluoroacetic acid containing 20 mM ammonium acetate allowed sufficient retention and separation of the glucosinolates in the sample extracts. Quadrupole time-of-flight tandem mass spectrometry in negative ion electrospray ionization was used to analyze the fractions collected from the HPLC elution to confirm the identification of the glucosinolates. The levels of sinigrin and gluconasturtiin in the vegetables and Chinese herbs were determined by using an external calibration method. Concentrations of gluconasturtiin in the Chinese herbs were more than 15 times higher than those of sinigrin. Detection limits were 18 nmol g-1 for sinigrin and 4nmol g-1 for gluconasturtiin when 50 g of fresh vegetable was analyzed.

AB - A reversed-phase HPLC method was developed for analyzing sinigrin and gluconasturtiin in six vegetable and two Chinese herb samples. A gradient program and mobile phases using methanol and 0.05% trifluoroacetic acid containing 20 mM ammonium acetate allowed sufficient retention and separation of the glucosinolates in the sample extracts. Quadrupole time-of-flight tandem mass spectrometry in negative ion electrospray ionization was used to analyze the fractions collected from the HPLC elution to confirm the identification of the glucosinolates. The levels of sinigrin and gluconasturtiin in the vegetables and Chinese herbs were determined by using an external calibration method. Concentrations of gluconasturtiin in the Chinese herbs were more than 15 times higher than those of sinigrin. Detection limits were 18 nmol g-1 for sinigrin and 4nmol g-1 for gluconasturtiin when 50 g of fresh vegetable was analyzed.

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Determination of two intact glucosinolates in vegetables and Chinese herbs

Abstract

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