胡桃楸的根、茎枝、叶和果皮中总鞣质的含量测定

目的: 建立胡桃楸中总鞣质的含量测定方法, 对不同地区采收的胡桃楸根、茎枝、叶和果皮中的鞣质含量进行比较分析。方法: 采用70%的丙酮超声提取样品中总鞣质, 以没食子酸为对照品, 磷钼钨酸试液为显色剂, 干酪素为鞣质吸附剂, 采用分光光度法于760 nm 处分别测定样品溶液中总多酚和游离多酚的含量, 从而计算出样品中总鞣质的含量。同时, 对鞣质含量测定中Na2CO3的用量、显色时间和干酪素的用量进行了考察。结果: 通过对鞣质含量测定各个环节的考察, 确定了胡桃楸中总鞣质的含量测定方法。该方法对鞣质的提取率高, 没食子酸在1.00~8.00 mg.L-1线性关系良好, r=0.999 7(n=5), 平均加样回收率为99.02%, RSD 3.7%(n=9); 大连和鞍山两地采集的胡桃楸根、茎枝、叶和果皮中鞣质的平均质量分数分别为45.66,23.40,58.24,3.58 mg.g-1。结论: 大连和鞍山2个产地的胡桃楸相应部位中鞣质含量非常一致, 2个产地的测定结果均说明胡桃楸不同药用部位中鞣质含量明显不同, 总鞣质含量的顺序为叶>根>茎枝>果皮。

The roots, barks, branches and pericarps of Juglans mandshurica were used as folk medicine in China and reputed for its' treatment of several cancers, such as gastric cancer, liver cancer and leukemia.The extracts of the roots, branches, leaves and pericarps of J.mandshurica have been experimentally proved to show anti-tumor activities.Tannins, which exhibited antioxidant and anti-tumor activities,were the main constituents in J.mandshurica. In this paper, a simple spectrophotometric method was developed for the determination of total tannins in the roots, branches, leaves and pericarps of J.mandshurica collected in Dalian and Anshan of Liaoning Province.Gallic acid was used as standard compound and the content of total tannins was calculated as gallic acid equivalent. As a result of the method validation, a good linearity(r=0.999 7, n=5) and a high recovery of gallic acid(99.02%, RSD 3.7%, n=9) was achieved.Eight samples including four parts of J.mandshurica collected in two places were analyzed for their total tannins with the established method. In the corresponding parts of J.mandshurica, except the pericarps, the contents of total tannins showed no significant difference between samples collected in Dalian and Anshan, while the content of total tannins in different parts of J.mandshurica were significantly different.The average content of total tannins in the roots, branches, leaves and pericarps of samples collected in Dalian and Anshan was 45.66, 23.40, 58.24, 3.58 mg·g-1, respectively.