Determination of Squalene Using High-Performance Liquid Chromatography with Diode Array Detection

Hai-Tao Lu; Yue Jiang; Feng Chen

doi:10.1365/s10337-003-0176-6

Determination of Squalene Using High-Performance Liquid Chromatography with Diode Array Detection

Hai-Tao Lu, Yue Jiang^*, Feng Chen

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

37 Citations (Scopus)

Abstract

A high-performance liquid chromatographic method with photodiode array detection has been developed for the determination of squalene. After treated by extraction and fractional crystallization, squalene was analyzed on a C18 column (150 × 3.9 mm, 5 μm) with acetonitrile as mobile phase. Excellent linearity of the calibration curve was observed in the range of 100–40000 μgL−1 and the detection limit was 40 μgL−1. The recoveries were from 89.6% to 100.5% and the relative standard deviations were from 0.5% to 1.4%. The method was successfully applied to the determination of squalene in squalene capsules, olive oil, algal lipids and algal cells.

Original language	English
Pages (from-to)	367–371
Number of pages	5
Journal	Chromatographia
Volume	59
Issue number	5-6
Early online date	6 Feb 2004
DOIs	https://doi.org/10.1365/s10337-003-0176-6
Publication status	Published - Mar 2004
Externally published	Yes

User-Defined Keywords

Column liquid chromatography
Extraction and fractional crystallization
Squalene from natural and pharmaceutical sources

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10.1365/s10337-003-0176-6

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title = "Determination of Squalene Using High-Performance Liquid Chromatography with Diode Array Detection",

abstract = "A high-performance liquid chromatographic method with photodiode array detection has been developed for the determination of squalene. After treated by extraction and fractional crystallization, squalene was analyzed on a C18 column (150 × 3.9 mm, 5 μm) with acetonitrile as mobile phase. Excellent linearity of the calibration curve was observed in the range of 100–40000 μgL−1 and the detection limit was 40 μgL−1. The recoveries were from 89.6% to 100.5% and the relative standard deviations were from 0.5% to 1.4%. The method was successfully applied to the determination of squalene in squalene capsules, olive oil, algal lipids and algal cells.",

keywords = "Column liquid chromatography, Extraction and fractional crystallization, Squalene from natural and pharmaceutical sources",

author = "Hai-Tao Lu and Yue Jiang and Feng Chen",

year = "2004",

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doi = "10.1365/s10337-003-0176-6",

language = "English",

volume = "59",

pages = "367–371",

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TY - JOUR

T1 - Determination of Squalene Using High-Performance Liquid Chromatography with Diode Array Detection

AU - Lu, Hai-Tao

AU - Jiang, Yue

AU - Chen, Feng

PY - 2004/3

Y1 - 2004/3

N2 - A high-performance liquid chromatographic method with photodiode array detection has been developed for the determination of squalene. After treated by extraction and fractional crystallization, squalene was analyzed on a C18 column (150 × 3.9 mm, 5 μm) with acetonitrile as mobile phase. Excellent linearity of the calibration curve was observed in the range of 100–40000 μgL−1 and the detection limit was 40 μgL−1. The recoveries were from 89.6% to 100.5% and the relative standard deviations were from 0.5% to 1.4%. The method was successfully applied to the determination of squalene in squalene capsules, olive oil, algal lipids and algal cells.

AB - A high-performance liquid chromatographic method with photodiode array detection has been developed for the determination of squalene. After treated by extraction and fractional crystallization, squalene was analyzed on a C18 column (150 × 3.9 mm, 5 μm) with acetonitrile as mobile phase. Excellent linearity of the calibration curve was observed in the range of 100–40000 μgL−1 and the detection limit was 40 μgL−1. The recoveries were from 89.6% to 100.5% and the relative standard deviations were from 0.5% to 1.4%. The method was successfully applied to the determination of squalene in squalene capsules, olive oil, algal lipids and algal cells.

KW - Column liquid chromatography

KW - Extraction and fractional crystallization

KW - Squalene from natural and pharmaceutical sources

UR - https://link.springer.com/article/10.1365/s10337-003-0176-6

U2 - 10.1365/s10337-003-0176-6

DO - 10.1365/s10337-003-0176-6

M3 - Journal article

SN - 0009-5893

VL - 59

SP - 367

EP - 371

JO - Chromatographia

JF - Chromatographia

IS - 5-6

ER -

Determination of Squalene Using High-Performance Liquid Chromatography with Diode Array Detection

Abstract

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