Determination of Squalene Using High-Performance Liquid Chromatography with Diode Array Detection

Hai-Tao Lu, Yue Jiang*, Feng Chen

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

36 Citations (Scopus)


A high-performance liquid chromatographic method with photodiode array detection has been developed for the determination of squalene. After treated by extraction and fractional crystallization, squalene was analyzed on a C18 column (150 × 3.9 mm, 5 μm) with acetonitrile as mobile phase. Excellent linearity of the calibration curve was observed in the range of 100–40000 μgL−1 and the detection limit was 40 μgL−1. The recoveries were from 89.6% to 100.5% and the relative standard deviations were from 0.5% to 1.4%. The method was successfully applied to the determination of squalene in squalene capsules, olive oil, algal lipids and algal cells.
Original languageEnglish
Pages (from-to)367–371
Number of pages5
Issue number5-6
Early online date6 Feb 2004
Publication statusPublished - Mar 2004
Externally publishedYes

User-Defined Keywords

  • Column liquid chromatography
  • Extraction and fractional crystallization
  • Squalene from natural and pharmaceutical sources


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