TY - JOUR
T1 - Determination of puerarin in pharmaceutical and biological samples by capillary zone electrophoresis with UV detection
AU - Liu, Lizhen
AU - Feng, Feng
AU - Shuang, Shaomin
AU - Bai, Yunfeng
AU - Choi, Martin M.F.
N1 - Funding Information:
Financial supports from the National Natural Science Foundation of China ( 21175085 ) and Natural Science Foundation of Shanxi Province ( 2009011015-1 ) are gratefully acknowledged.
PY - 2012/3/15
Y1 - 2012/3/15
N2 - A rapid and efficient capillary zone electrophoresis (CZE) method with ultraviolet detection has been successfully developed for the determination of puerarin in Xinkeshu capsules and biological samples. The optimal CZE conditions were determined to be 70 mM H3BO3-Na2B 4O7, pH 9.2, injection time 5 s, applied voltage 25 kV, working temperature 25 °C and detection wavelength 254 nm. Under these conditions, a linear range from 17.3 to 138 μg/mL with the correlation coefficient of 0.9998 and limit of detection of 34.6 ng/mL (S/N = 3) for puerarin were obtained. The relative standard deviation of the migration time and peak area of puerarin were 0.23-0.91, 0.43-1.62, 0.10-0.64 and 0.64-1.32% for intra-day and inter-day analyses, respectively. Satisfactory results were obtained for recovery and repeatability. The proposed method was successfully applied to analyze the puerarin content in Xinkeshu capsules and some biological samples with good results. It is suitable for applications in pharmaceutical industries for quality control and in clinical laboratories for therapeutic drug monitoring purposes.
AB - A rapid and efficient capillary zone electrophoresis (CZE) method with ultraviolet detection has been successfully developed for the determination of puerarin in Xinkeshu capsules and biological samples. The optimal CZE conditions were determined to be 70 mM H3BO3-Na2B 4O7, pH 9.2, injection time 5 s, applied voltage 25 kV, working temperature 25 °C and detection wavelength 254 nm. Under these conditions, a linear range from 17.3 to 138 μg/mL with the correlation coefficient of 0.9998 and limit of detection of 34.6 ng/mL (S/N = 3) for puerarin were obtained. The relative standard deviation of the migration time and peak area of puerarin were 0.23-0.91, 0.43-1.62, 0.10-0.64 and 0.64-1.32% for intra-day and inter-day analyses, respectively. Satisfactory results were obtained for recovery and repeatability. The proposed method was successfully applied to analyze the puerarin content in Xinkeshu capsules and some biological samples with good results. It is suitable for applications in pharmaceutical industries for quality control and in clinical laboratories for therapeutic drug monitoring purposes.
KW - Biological samples
KW - Capillary zone electrophoresis
KW - Puerarin
KW - Xinkeshu capsules
UR - http://www.scopus.com/inward/record.url?scp=84862789090&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2012.01.022
DO - 10.1016/j.talanta.2012.01.022
M3 - Journal article
AN - SCOPUS:84862789090
SN - 0039-9140
VL - 91
SP - 83
EP - 87
JO - Talanta
JF - Talanta
ER -