TY - JOUR
T1 - Determination of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls in human serum using programmable-temperature vaporization gas chromatography with high-resolution mass spectrometry
AU - Zhang, Lei
AU - Zhong, Yuxin
AU - Liu, Xin
AU - Bao, Yan
AU - Zhao, Yunfeng
AU - Wu, Yongning
AU - Cai, Zongwei
AU - Li, Jingguang
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (grant numbers 21507018, 21477030, 21537001, 21361140359), and Youth Research Fund of China National Center for Food Safety Risk Assessment (CFSA) (grant number 2015001).
PY - 2017/9
Y1 - 2017/9
N2 - The determination of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls in blood from a non-occupational population is essential for the investigation of adverse health effects from these pollutants. In this study, a sensitive method based on programmable-temperature vaporization with large-volume injection coupled with gas chromatography with high-resolution mass spectrometry was developed to determine these pollutants in 1–2 mL of human serum samples. Various key parameters of programmable-temperature vaporization injector, including vent temperature, vent time, vent flow, transfer temperature and transfer time were optimized by factorial design. The accuracy and precision as well as applicability were assessed by determining polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls in calibration standard solutions, standard reference materials and real human serum samples from non-occupational population. The method detection limits of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls were 1.5–9.0 and 0.005–0.02 ng/kg wet weight, respectively. By comparing with typically splitless injection, the application of programmable-temperature vaporization injector could effectively lead to higher detectable rate of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls in 1–2 mL of human serum samples.
AB - The determination of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls in blood from a non-occupational population is essential for the investigation of adverse health effects from these pollutants. In this study, a sensitive method based on programmable-temperature vaporization with large-volume injection coupled with gas chromatography with high-resolution mass spectrometry was developed to determine these pollutants in 1–2 mL of human serum samples. Various key parameters of programmable-temperature vaporization injector, including vent temperature, vent time, vent flow, transfer temperature and transfer time were optimized by factorial design. The accuracy and precision as well as applicability were assessed by determining polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls in calibration standard solutions, standard reference materials and real human serum samples from non-occupational population. The method detection limits of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls were 1.5–9.0 and 0.005–0.02 ng/kg wet weight, respectively. By comparing with typically splitless injection, the application of programmable-temperature vaporization injector could effectively lead to higher detectable rate of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls in 1–2 mL of human serum samples.
KW - dioxins
KW - human serum
KW - large-volume injection
KW - programmable-temperature vaporization
UR - http://www.scopus.com/inward/record.url?scp=85026439507&partnerID=8YFLogxK
U2 - 10.1002/jssc.201700465
DO - 10.1002/jssc.201700465
M3 - Journal article
C2 - 28665025
AN - SCOPUS:85026439507
SN - 1615-9306
VL - 40
SP - 3453
EP - 3461
JO - Journal of Separation Science
JF - Journal of Separation Science
IS - 17
ER -