Determination of paralytic shellfish toxins in dinoflagellate Alexandrium tamarense by using isotachophoresis/capillary electrophoresis

Youyi Wu, Alvin Yam Tat Ho, Pei Yuan Qian, Kelvin S Y LEUNG, Zongwei CAI*, Jin Ming Lin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Baseline separation of seven paralytic shellfish toxins (PSTs), namely decarbamoylsaxitoxin (dcSTX), saxitoxin (STX), neosaxitoxin (NEO), gonyautoxin-2 (GTX-2), gonyautoxin-3 (GTX-3), gonyautoxin-1 (GTX-1), and gonyautoxin-4 (GTX-4), was achieved by using capillary ITP (CITP)/CE with UV detection. Separation parameters including duration time and voltage in CITP process, separation voltage, and pH and concentration of buffer were optimized. The developed method provided linear responses from 1.3 to 200 μM for the PSTs. The LOD ranged from 0.1 to 0.3 μM. PST extracts from two algal strains of Alexandrium tamarense were analyzed and the toxin concentrations in the samples were quantified with an internal standard method by using NEO as the internal standard. The algal extract of A. tamarense HK9301 contained 332 μM GTX-2 and 224 μM GTX-3, while the PSTs were not detected in the extract of A. tamarense CI01.

Original languageEnglish
Pages (from-to)399-404
Number of pages6
JournalJournal of Separation Science
Volume29
Issue number3
DOIs
Publication statusPublished - Feb 2006

Scopus Subject Areas

  • Analytical Chemistry
  • Filtration and Separation

User-Defined Keywords

  • Alexandrium tamarense extracts
  • Capillary electrophoresis
  • Isotachophoresis
  • Paralytic shellfish toxins

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