TY - JOUR
T1 - Determination of mycobiota and aflatoxin contamination in commercial bee pollen from eight provinces and one autonomous region of China
AU - Guo, Mengyue
AU - Zhang, Jing
AU - Wang, Yunyun
AU - Chen, Hubiao
AU - Lv, Jianxin
AU - Kong, Dandan
AU - Jin, Ziyue
AU - Ke, Tongwei
AU - Zhang, Hongkun
AU - Luo, Jiaoyang
AU - Yang, Meihua
N1 - This work was supported by the National Natural Science Foundation of China ( 82204618 ) and the CAMS Innovation Fund for Medical Sciences ( 2021-I2M-1-071 ), the High-end Foreign Experts Recruitment Plan of China ( G2022194012L ), and NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine ( 2023GSMPA-KL19 ).
Publisher Copyright:
© 2023 Elsevier B.V. All rights reserved.
PY - 2024/2/2
Y1 - 2024/2/2
N2 - The co-occurrence of fungi and mycotoxins in various foods has been frequently reported in many countries, posing a serious threat to the health and safety of consumers. In this study, the mycobiota in five types of commercial bee pollen samples from China were first revealed by DNA metabarcoding. Meanwhile, the content of total aflatoxins in each sample was investigated by high-performance liquid chromatography with fluorescence detection. The results demonstrated that Cladosporium (0.16 %–89.29 %) was the most prevalent genus in bee pollen, followed by Metschnikowia (0–81.12 %), unclassified genus in the phylum Ascomycota (0–81.13 %), Kodamaea (0–73.57 %), and Penicillium (0–36.13 %). Meanwhile, none of the assayed aflatoxins were determined in the 18 batches of bee pollen samples. In addition, the fungal diversity, community composition, and trophic mode varied significantly among five groups. This study provides comprehensive information for better understanding the fungal communities and aflatoxin residues in bee pollen from different floral origins in China.
AB - The co-occurrence of fungi and mycotoxins in various foods has been frequently reported in many countries, posing a serious threat to the health and safety of consumers. In this study, the mycobiota in five types of commercial bee pollen samples from China were first revealed by DNA metabarcoding. Meanwhile, the content of total aflatoxins in each sample was investigated by high-performance liquid chromatography with fluorescence detection. The results demonstrated that Cladosporium (0.16 %–89.29 %) was the most prevalent genus in bee pollen, followed by Metschnikowia (0–81.12 %), unclassified genus in the phylum Ascomycota (0–81.13 %), Kodamaea (0–73.57 %), and Penicillium (0–36.13 %). Meanwhile, none of the assayed aflatoxins were determined in the 18 batches of bee pollen samples. In addition, the fungal diversity, community composition, and trophic mode varied significantly among five groups. This study provides comprehensive information for better understanding the fungal communities and aflatoxin residues in bee pollen from different floral origins in China.
KW - Aflatoxins
KW - Bee pollen
KW - DNA metabarcoding
KW - HPLC-FLD
KW - Mycobiota
KW - Mycotoxins
UR - http://www.scopus.com/inward/record.url?scp=85178551911&partnerID=8YFLogxK
U2 - 10.1016/j.ijfoodmicro.2023.110511
DO - 10.1016/j.ijfoodmicro.2023.110511
M3 - Journal article
C2 - 38043476
AN - SCOPUS:85178551911
SN - 0168-1605
VL - 411
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
M1 - 110511
ER -
