Abstract
Herba Oldenlandiae, the dried herb of Oldenlandia diffusa (WILLD.) ROXB. (Family Rubiaceae), is officially listed in the Chinese Pharmacopoeia. In the herbal market, two substitutes originated from O. corymbosa (L.) LAM and O. tenelliflora BL.
are commonly used. In light of this, the target in setting up a method
for quality assessment of Herba Oldenlandiae is urgently needed. In this
article, a simple and reliable high-performance liquid chromatographic
(HPLC) method was developed for quantifying asperuloside (1), E-6-O-p-coumaroyl scandoside methyl ester (2) and E-6-O-p-coumaroyl scandoside methyl ester-10-methyl ether (3) in Herba Oldenlandiae derived from O. diffusa. Among them, compound 3 is a new compound isolated from O. diffusa.
All these unique compounds were used as markers for the first time in
the quality assessment of Herba Oldenlandiae. The results showed that
the contents of compounds 1—3 were significantly varied among different
samples whilst those of compounds 2 and 3 were found to be lower in
contents in the two substitutes of O. diffusa. The analytical
method is suitable for quality control of Herba Oldenlandiae and useful
in differentiation from its confusable species. The method has been
fully validated with satisfactory linearity, accuracy, precision and
stability.
| Original language | English |
|---|---|
| Pages (from-to) | 1131-1137 |
| Number of pages | 7 |
| Journal | Chemical and Pharmaceutical Bulletin |
| Volume | 54 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Aug 2006 |
Scopus Subject Areas
- General Chemistry
- Drug Discovery
User-Defined Keywords
- Oldenlandia
- iridoid glucoside
- Herba Oldenlandiae
- quality control
- HPLC