Determination of glucosinolates in traditional Chinese herbs by high-performance liquid chromatography and electrospray ionization mass spectrometry

Kim Chung Lee, Man Wai Cheuk, Wan Chan, Albert W M LEE, Zhongzhen ZHAO, Zhi Hong JIANG, Zongwei CAI*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

A reversed-phase HPLC method has been developed for determination of twelve intact glucosinolates-glucoiberin, glucocheirolin, progoitrin, sinigrin, epiprogoitrin, glucoraphenin, sinalbin, gluconapin, glucosibarin, glucotropaeolin, glucoerucin, and gluconasturtiin-in ten traditional Chinese plants. The samples were extracted with methanol and the extracts were cleaned on an activated Florisil column. A mobile phase gradient prepared from methanol and 30 mmol L-1 ammonium acetate at pH 5.0 enabled baseline separation of the glucosinolates. Glucosinolate detection was confirmed by quadrupole time-of-flight tandem mass spectrometric analysis in negative-ionization mode. Detection limits ranged from 0.06 to 0.36 μg g -1 when 5 g of dried plant was analyzed. Recoveries of the glucosinolates were better than 85% and precision (relative standard derivation, n∈=∈3) ranged from 5.3 to 14.6%. Analysis of the glucosinolates provided scientific evidence enabling differentiation of three pairs of easily confused plants.

Original languageEnglish
Pages (from-to)2225-2232
Number of pages8
JournalAnalytical and Bioanalytical Chemistry
Volume386
Issue number7-8
DOIs
Publication statusPublished - Dec 2006

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry

User-Defined Keywords

  • ESI-MS
  • Glucosinolate
  • HPLC
  • Traditional Chinese herb

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