Determination of glucose in human serum based on an onion primary cuticula biosensor immobilized glucose oxidase

Wenjuan Jia, Wenjuan Liu, Yan Zhang, Miao Cui, Shaomin Shuang*, Chuan Dong, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A glucose biosensor based on onion primary cuticula immobilized glucose oxidase has been developed. The glucose concentration was quantified by the decrease of dissolved oxygen. The effects of enzyme loading, pH, temperature, buffer concentration and interference have been studied in detail. The biosensor showed a wide linear relationship with a concentration range of 20 μM-1.1 mM, a fast response time (70 s), good repeatability (2.8%, n = 10) and satisfactory reproducibility (0.91%, n = 3). The biosensor retained 90% of its initial response after 3 months at 25 °C. The apparent Michaelis-Menten constant K m was estimated to be 0.042 mM, which revealed higher biological affinity to glucose. The proposed biosensor method has been successfully applied to the determination of glucose concentration in human blood serum and obtained results were agreement with the colorimetric assay kit. The recoveries of proposed method are 95.2-106.3%.

Original languageEnglish
Pages (from-to)1432-1437
Number of pages6
JournalAnalytical Methods
Volume4
Issue number5
DOIs
Publication statusPublished - May 2012

Scopus Subject Areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

Fingerprint

Dive into the research topics of 'Determination of glucose in human serum based on an onion primary cuticula biosensor immobilized glucose oxidase'. Together they form a unique fingerprint.

Cite this