Determination of five nitroimidazole residues in artificial porcine muscle tissue samples by capillary electrophoresis

Yingyun Lin, Yan Su, Xiulin Liao, Na Yang, Xiupei Yang*, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

A capillary electrophoresis (CE) method with ultraviolet detection has been developed for simultaneous detection and quantification of five nitroimidazoles including benzoylmetronidazole, dimetridazole, metronidazole, ronidazole, and secnidazole in porcine muscles. Nitroimidazoles in samples were extracted by ethyl acetate with subsequent clean-up by a strong cation exchange solid phase extraction column. The clean extracts were subjected to CE separation with optimal experimental conditions: pH 3.0 running buffer containing 25 mM sodium phosphate and 0.10 mM tetrabutylammonium bromide, 5 s hydrodynamic injection at 0.5 psi and 28 kV separation voltage. The nitroimidazoles could be monitored and detected at 320 nm within 18 min. The limits of detection were below 1.0 μg/kg and limits of quantification were lower than 3.2 μg/kg for all nitroimidazoles in the muscle samples. The recoveries and relative standard deviations were 85.4-96.0, 83.5-92.5, 1.3-3.9, and 1.1-4.2%, respectively for the intra-day and inter-day analyses. The proposed CE method has been successfully applied to determine nitroimidazoles in artificial porcine muscle samples with good accuracy and recovery, demonstrating that it has potential for detection and quantification of multi-nitroimidazole residue in real muscle samples.

Original languageEnglish
Pages (from-to)646-652
Number of pages7
JournalTalanta
Volume88
DOIs
Publication statusPublished - 15 Jan 2012

Scopus Subject Areas

  • Analytical Chemistry

User-Defined Keywords

  • Capillary electrophoresis
  • Nitroimidazole
  • Porcine tissues
  • Residues

Fingerprint

Dive into the research topics of 'Determination of five nitroimidazole residues in artificial porcine muscle tissue samples by capillary electrophoresis'. Together they form a unique fingerprint.

Cite this