Determination of dehydroevodiamine in Evodia rutaecarpa (Juss.) Benth by high performance liquid chromatography and classification of the samples by using hierarchical clustering analysis

Yang Zhao, Xin Zhou*, Hua Guo Chen, Xiao Jian Gong, Zongwei CAI, Chan Yuan Zhou

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

A simple, sensitive and accurate liquid chromatographic method with photodiode-array detection was developed for determination of dehydroevodiamine with detection wavelength at 368 nm and column temperature at 30 °C. The separation was carried out on an Agilent Zorbax SB-C18 column (250 mm × 4.6 mm, 5 μm) together with a C18 guard column. The mobile phase was acetonitrile-water (containing 30 mM sodium acetate trihydrate and 0.15% acetic acid) in the ratio of 30:70 (v/v) delivered at a flow rate of 1 mL/min. Excellent linear behavior was observed over the concentration range investigated, with correlation coefficient (R2) = 0.9998. This validated method was applied to determine the contents of dehydroevodiamine in 36 samples from different regions of China, and hierarchical clustering analysis was firstly used to classify and differentiate Evodia rutaecarpa samples. The analysis is specific and can be successfully applied to analyze E. rutaecarpa which is helpful for quality control of the herb.

Original languageEnglish
Pages (from-to)415-420
Number of pages6
JournalFitoterapia
Volume80
Issue number7
DOIs
Publication statusPublished - Oct 2009

Scopus Subject Areas

  • Pharmacology
  • Drug Discovery

User-Defined Keywords

  • Dehydroevodiamine
  • Evodia rutaecarpa (Juss.) Benth
  • Hierarchical clustering analysis
  • High performance liquid chromatography

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