Determination of cell metabolite VEGF165 and dynamic analysis of protein-DNA interactions by combination of microfluidic technique and luminescent switch-on probe

Xuexia Lin, Ka Ho Leung, Ling Lin, Luyao Lin, Sheng Lin, Chung Hang Leung, Edmond Dik Lung MA*, Jin Ming Lin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)

Abstract

In this paper, we rationally design a novel G-quadruplex-selective luminescent iridium (III) complex for rapid detection of oligonucleotide and VEGF165 in microfluidics. This new probe is applied as a convenient biosensor for label-free quantitative analysis of VEGF165 protein from cell metabolism, as well as for studying the kinetics of the aptamer-protein interaction combination with a microfluidic platform. As a result, we have successfully established a quantitative analysis of VEGF165 from cell metabolism. Furthermore, based on the principles of hydrodynamic focusing and diffusive mixing, different transient states during kinetics process were monitored and recorded. Thus, the combination of microfluidic technique and G-quadruplex luminescent probe will be potentially applied in the studies of intramolecular interactions and molecule recognition in the future.

Original languageEnglish
Pages (from-to)41-47
Number of pages7
JournalBiosensors and Bioelectronics
Volume79
DOIs
Publication statusPublished - 15 May 2016

Scopus Subject Areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

User-Defined Keywords

  • Functional nucleic acid
  • Iridium (III) probe
  • Kinetic analysis
  • Microfluidic device
  • VEGF

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