TY - JOUR
T1 - Determination of benzotriazoles and benzothiazoles in human urine by UHPLC-TQMS
AU - Li, Jiufeng
AU - Zhao, Hongzhi
AU - Zhou, Yanqiu
AU - Xu, Shunqing
AU - CAI, Zongwei
N1 - Funding Information:
This work was supported by National Natural Science Foundation of China (NO. 21437002 ) and Collaborative Research Fund (CRF) from Research Grants Council of Hong Kong (C2014-14E).
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Benzotriazole (BTR) and benzothiazole (BTH) derivatives are extensively applied in industrial processes and consumer products, and are thus frequently detected in the environmental matrices. Due to their potential estrogenic effects reported in animal studies, the assessment of human exposure to BTRs and BTHs is important. In this study, a method was developed and validated to determine six BTRs and five BTHs in human urine by using ultra-high performance liquid chromatography coupled with a triple quadrupole mass spectrometry (UHPLC-TQMS) in positive electrospray ionization mode. After de-conjugation by β-glucuronidase, urine samples were liquid-liquid extracted for the measurement of total concentrations of BTRs and BTHs. The linearity, detection limit, precision, recovery, accuracy and matrix effect were evaluated. The limits of detection were less than 0.5 ng/mL. The validated method demonstrated good precision (RSD% < 15%), linearity (R2 > 0.99), recovery (>80%) and accuracy (80–100%). The method was successfully applied for the analysis of 22 human urine samples. Four out of eleven targeted compounds were detected in more than half of participants at ng/mL levels.
AB - Benzotriazole (BTR) and benzothiazole (BTH) derivatives are extensively applied in industrial processes and consumer products, and are thus frequently detected in the environmental matrices. Due to their potential estrogenic effects reported in animal studies, the assessment of human exposure to BTRs and BTHs is important. In this study, a method was developed and validated to determine six BTRs and five BTHs in human urine by using ultra-high performance liquid chromatography coupled with a triple quadrupole mass spectrometry (UHPLC-TQMS) in positive electrospray ionization mode. After de-conjugation by β-glucuronidase, urine samples were liquid-liquid extracted for the measurement of total concentrations of BTRs and BTHs. The linearity, detection limit, precision, recovery, accuracy and matrix effect were evaluated. The limits of detection were less than 0.5 ng/mL. The validated method demonstrated good precision (RSD% < 15%), linearity (R2 > 0.99), recovery (>80%) and accuracy (80–100%). The method was successfully applied for the analysis of 22 human urine samples. Four out of eleven targeted compounds were detected in more than half of participants at ng/mL levels.
KW - Benzothiazoles
KW - Benzotriazoles
KW - Human urine analysis
KW - Liquid chromatography-tandem mass spectrometry
KW - Liquid-liquid extraction
UR - http://www.scopus.com/inward/record.url?scp=85032341169&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2017.10.045
DO - 10.1016/j.jchromb.2017.10.045
M3 - Article
C2 - 29089228
AN - SCOPUS:85032341169
SN - 1570-0232
VL - 1070
SP - 70
EP - 75
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ER -