Determination of a potential antitumor quassinoid in rat plasma by UPLC-MS/MS and its application in a pharmacokinetic study

Qiang Zhang; Yonghui Yuan; Jianchun Cui; Tingting Xiao; Zhipeng Deng; Daqing Jiang

doi:10.1016/j.jpba.2016.02.046

Determination of a potential antitumor quassinoid in rat plasma by UPLC-MS/MS and its application in a pharmacokinetic study

Qiang Zhang, Yonghui Yuan, Jianchun Cui, Tingting Xiao, Zhipeng Deng^*, Daqing Jiang^*

^*Corresponding author for this work

Chinese Medicine - Teaching and Research Division

Research output: Contribution to journal › Journal article › peer-review

9 Citations (Scopus)

Abstract

A sensitive UPLC-MS/MS method was developed and validated for the determination of brusatol in rat plasma. Chromatographic separation was carried out on a C₁₈ column using methanol and 10 mM ammonium acetate containing 0.1% (v/v) formic acid (55:45, v/v). The lower limit of quantification (LLOQ) was 1.0 ng/mL for brusatol in plasma. The intra- and inter-day precision for the analyte ranged from 3.2% to 9.2% and 1.3% to 7.8%, and the accuracy was between 97.3% and 108.5%. The method was successfully applied in a pharmacokinetic study of brusatol following intravenous injection (0.5, 1.0, and 2.0 mg/kg) of brusatol.

Original language	English
Pages (from-to)	143-148
Number of pages	6
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	124
DOIs	https://doi.org/10.1016/j.jpba.2016.02.046
Publication status	Published - 30 May 2016

User-Defined Keywords

Brusatol
Pharmacokinetic study
Quassinoid
Rat plasma
UPLC-MS/MS

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10.1016/j.jpba.2016.02.046

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title = "Determination of a potential antitumor quassinoid in rat plasma by UPLC-MS/MS and its application in a pharmacokinetic study",

abstract = "A sensitive UPLC-MS/MS method was developed and validated for the determination of brusatol in rat plasma. Chromatographic separation was carried out on a C18 column using methanol and 10 mM ammonium acetate containing 0.1% (v/v) formic acid (55:45, v/v). The lower limit of quantification (LLOQ) was 1.0 ng/mL for brusatol in plasma. The intra- and inter-day precision for the analyte ranged from 3.2% to 9.2% and 1.3% to 7.8%, and the accuracy was between 97.3% and 108.5%. The method was successfully applied in a pharmacokinetic study of brusatol following intravenous injection (0.5, 1.0, and 2.0 mg/kg) of brusatol.",

keywords = "Brusatol, Pharmacokinetic study, Quassinoid, Rat plasma, UPLC-MS/MS",

author = "Qiang Zhang and Yonghui Yuan and Jianchun Cui and Tingting Xiao and Zhipeng Deng and Daqing Jiang",

note = "This work was financially supported by the National Natural Science Foundation of Shandong Province (ZR2015YL048), and Jieping Wu Medical Foundation (320.6750.14070). Publisher Copyright: {\textcopyright} 2016 Elsevier B.V. All rights reserved.",

year = "2016",

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day = "30",

doi = "10.1016/j.jpba.2016.02.046",

language = "English",

volume = "124",

pages = "143--148",

journal = "Journal of Pharmaceutical and Biomedical Analysis",

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TY - JOUR

T1 - Determination of a potential antitumor quassinoid in rat plasma by UPLC-MS/MS and its application in a pharmacokinetic study

AU - Zhang, Qiang

AU - Yuan, Yonghui

AU - Cui, Jianchun

AU - Xiao, Tingting

AU - Deng, Zhipeng

AU - Jiang, Daqing

N1 - This work was financially supported by the National Natural Science Foundation of Shandong Province (ZR2015YL048), and Jieping Wu Medical Foundation (320.6750.14070). Publisher Copyright: © 2016 Elsevier B.V. All rights reserved.

PY - 2016/5/30

Y1 - 2016/5/30

N2 - A sensitive UPLC-MS/MS method was developed and validated for the determination of brusatol in rat plasma. Chromatographic separation was carried out on a C18 column using methanol and 10 mM ammonium acetate containing 0.1% (v/v) formic acid (55:45, v/v). The lower limit of quantification (LLOQ) was 1.0 ng/mL for brusatol in plasma. The intra- and inter-day precision for the analyte ranged from 3.2% to 9.2% and 1.3% to 7.8%, and the accuracy was between 97.3% and 108.5%. The method was successfully applied in a pharmacokinetic study of brusatol following intravenous injection (0.5, 1.0, and 2.0 mg/kg) of brusatol.

AB - A sensitive UPLC-MS/MS method was developed and validated for the determination of brusatol in rat plasma. Chromatographic separation was carried out on a C18 column using methanol and 10 mM ammonium acetate containing 0.1% (v/v) formic acid (55:45, v/v). The lower limit of quantification (LLOQ) was 1.0 ng/mL for brusatol in plasma. The intra- and inter-day precision for the analyte ranged from 3.2% to 9.2% and 1.3% to 7.8%, and the accuracy was between 97.3% and 108.5%. The method was successfully applied in a pharmacokinetic study of brusatol following intravenous injection (0.5, 1.0, and 2.0 mg/kg) of brusatol.

KW - Brusatol

KW - Pharmacokinetic study

KW - Quassinoid

KW - Rat plasma

KW - UPLC-MS/MS

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U2 - 10.1016/j.jpba.2016.02.046

DO - 10.1016/j.jpba.2016.02.046

M3 - Journal article

C2 - 26945636

AN - SCOPUS:84959340396

SN - 0731-7085

VL - 124

SP - 143

EP - 148

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

ER -

Determination of a potential antitumor quassinoid in rat plasma by UPLC-MS/MS and its application in a pharmacokinetic study

Abstract

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