TY - JOUR
T1 - Determination of 14 heterocyclic aromatic amines in meat products using solid-phase extraction and supercritical fluid chromatography coupled to triple quadrupole mass spectrometry
AU - Zhang, Yuan
AU - Wu, Wen Jie
AU - Zhou, Wei E
AU - Ren, Zhi Qin
AU - Feng, Xue Song
AU - Zhang, Feng
N1 - Funding Information:
This work was financially supported by the National Key Research and Development Program of China (2017YFC1601600).
Publisher Copyright:
© 2020 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2020/4
Y1 - 2020/4
N2 - A novel, simple, and sensitive method has been developed for simultaneous determination of 14 heterocyclic aromatic amines in meat product using solid-phase extraction combined with ultrahigh-performance supercritical fluid chromatography coupled to tandem quadrupole mass spectrometry. The analytes could be separated within 7 min and identified using their retention times and mass. The developed method was validated based on the linearity, limits of quantification, precision, and accuracy. The recovery ranged from 52.3 to 97.5% with an acceptable standard deviation, which is not higher than 6%. The limits of quantitation ranged from 0.03 to 0.17 µg/kg. The selectivity and sensitivity were satisfactory in multiple reaction monitoring mode. The method was applied to commercial meat products, and the results demonstrated that the novel method has potential for the analysis of the targets in food matrices. This is the first work reporting the simultaneous quantification of 14 heterocyclic aromatic amines by means of ultrahigh-performance supercritical fluid chromatography coupled to tandem quadrupole mass spectrometry.
AB - A novel, simple, and sensitive method has been developed for simultaneous determination of 14 heterocyclic aromatic amines in meat product using solid-phase extraction combined with ultrahigh-performance supercritical fluid chromatography coupled to tandem quadrupole mass spectrometry. The analytes could be separated within 7 min and identified using their retention times and mass. The developed method was validated based on the linearity, limits of quantification, precision, and accuracy. The recovery ranged from 52.3 to 97.5% with an acceptable standard deviation, which is not higher than 6%. The limits of quantitation ranged from 0.03 to 0.17 µg/kg. The selectivity and sensitivity were satisfactory in multiple reaction monitoring mode. The method was applied to commercial meat products, and the results demonstrated that the novel method has potential for the analysis of the targets in food matrices. This is the first work reporting the simultaneous quantification of 14 heterocyclic aromatic amines by means of ultrahigh-performance supercritical fluid chromatography coupled to tandem quadrupole mass spectrometry.
KW - food-derived hazardous compounds
KW - heterocyclic aromatic amines
KW - meat
KW - supercritical fluid chromatography
KW - triple quadrupole mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=85083543915&partnerID=8YFLogxK
U2 - 10.1002/jssc.201900816
DO - 10.1002/jssc.201900816
M3 - Journal article
C2 - 31944578
AN - SCOPUS:85083543915
SN - 1615-9306
VL - 43
SP - 1372
EP - 1381
JO - Journal of Separation Science
JF - Journal of Separation Science
IS - 7
ER -