TY - JOUR
T1 - Detection of water toxicity using cytochrome P450 transgenic zebrafish as live biosensor
T2 - For polychlorinated biphenyls toxicity
AU - Hung, Karen W.Y.
AU - Suen, Miranda F.K.
AU - Chen, Y. F.
AU - Cai, H. B.
AU - Mo, Z. X.
AU - YUNG, Kin Lam
N1 - Funding Information:
The present study was supported by Innovation and Technology Fund, Hong Kong ITF/07-08/01 and ITF/09-10/03 and Mini Area of Excellence Scheme, Research Committee, Hong Kong Baptist University RC/AOE/08-09/02 .
PY - 2012/1/15
Y1 - 2012/1/15
N2 - Cytochrome P450 (CYPs) is significant in degradation of endogenous substrates and detoxification of carcinogens, therefore it is a biomarker for assessment of polycyclic aromatic hydrocarbons (PAHs) level in aquatic environment. In the present study, a transgenic line of zebrafish had been generated using a CYP-green fluorescence protein (CYP-GFP) construct, driven by CYP1A1 promoter. Polychlorinated biphenyls (PCBs) were used as toxicant, in concentrations of 0.02. μg/ml, 0.04. μg/ml, 0.08. μg/ml, 0.4. μg/ml, and 0.8. μg/ml. The transgenic control fish showed low intensity of fluorescence in the liver. After exposed to PCBs, zebrafish had morphological changes such as expansion of yolk, contortion of tails and inflation of pericardial area. Green fluorescence signals were found to express according to concentrations and time. The green fluorescence signal was most intense after treatment with 0.08. μg/ml PCBs. However, the maximum area of green fluorescent signal was found at 0.04. μg/ml PCBs. GFP started to express at 3. h exposure to PCBs, increasing its intensity until 6. h exposure, and then level off. Since the GFP expression is fast responding and is sensitive to low PAHs concentrations, transgenic fish is a good tool for live imaging and monitoring of aquatic contamination.
AB - Cytochrome P450 (CYPs) is significant in degradation of endogenous substrates and detoxification of carcinogens, therefore it is a biomarker for assessment of polycyclic aromatic hydrocarbons (PAHs) level in aquatic environment. In the present study, a transgenic line of zebrafish had been generated using a CYP-green fluorescence protein (CYP-GFP) construct, driven by CYP1A1 promoter. Polychlorinated biphenyls (PCBs) were used as toxicant, in concentrations of 0.02. μg/ml, 0.04. μg/ml, 0.08. μg/ml, 0.4. μg/ml, and 0.8. μg/ml. The transgenic control fish showed low intensity of fluorescence in the liver. After exposed to PCBs, zebrafish had morphological changes such as expansion of yolk, contortion of tails and inflation of pericardial area. Green fluorescence signals were found to express according to concentrations and time. The green fluorescence signal was most intense after treatment with 0.08. μg/ml PCBs. However, the maximum area of green fluorescent signal was found at 0.04. μg/ml PCBs. GFP started to express at 3. h exposure to PCBs, increasing its intensity until 6. h exposure, and then level off. Since the GFP expression is fast responding and is sensitive to low PAHs concentrations, transgenic fish is a good tool for live imaging and monitoring of aquatic contamination.
KW - CYP1A1
KW - Cytochrome P450
KW - GFP
KW - Polychlorinated biphenyls
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=84455202507&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2011.10.004
DO - 10.1016/j.bios.2011.10.004
M3 - Journal article
C2 - 22024592
AN - SCOPUS:84455202507
SN - 0956-5663
VL - 31
SP - 548
EP - 553
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 1
ER -