Detection of Newcastle disease virus using nucleic acid sequence-based amplification

Shang Jin Cui; Yin Wan Wendy Fung; Lok Ting Lau; Wen Bin Liu; Yun Feng Wang; Guang Zhi Tong; Jianguo Chen; Albert Cheung Hoi Yu

doi:10.1016/j.biologicals.2005.12.002

Detection of Newcastle disease virus using nucleic acid sequence-based amplification

Shang Jin Cui, Yin Wan Wendy Fung, Lok Ting Lau, Wen Bin Liu, Yun Feng Wang, Guang Zhi Tong, Jianguo Chen, Albert Cheung Hoi Yu^*

^*Corresponding author for this work

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Research output: Contribution to journal › Journal article › peer-review

6 Citations (Scopus)

Abstract

Newcastle disease (ND) is a contagious and widespread avian disease affecting most species of birds. ND virus (NDV) is the only member of the avian paramyxovirus serotype 1 (APMV1) causing ND outbreak in bird flocks. The technique of nucleic acid sequence-based amplification (NASBA) is a potential method to rapidly and reliably detect NDV isolates. Here, we describe an effective and unprecedented method for detecting NDV strains of all pathotypes. A conserved region of the fusion protein gene was used for designing oligonucleotides specific to all NDV pathotypes. The dynamic range of this NDV NASBA detection method is comparable to virus culture and therefore the NDV NASBA method is a potential alternative for NDV screening and surveillance.

Original language	English
Pages (from-to)	13-18
Number of pages	6
Journal	Biologicals
Volume	35
Issue number	1
DOIs	https://doi.org/10.1016/j.biologicals.2005.12.002
Publication status	Published - Mar 2007

Scopus Subject Areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
General Immunology and Microbiology
Pharmacology

User-Defined Keywords

Disease screening
NASBA
NDV
Newcastle disease
RNA amplicons
Surveillance

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biologicals.2005.12.002

Cite this

@article{c06e199911ac4a9eae0fc02bce1edb99,

title = "Detection of Newcastle disease virus using nucleic acid sequence-based amplification",

abstract = "Newcastle disease (ND) is a contagious and widespread avian disease affecting most species of birds. ND virus (NDV) is the only member of the avian paramyxovirus serotype 1 (APMV1) causing ND outbreak in bird flocks. The technique of nucleic acid sequence-based amplification (NASBA) is a potential method to rapidly and reliably detect NDV isolates. Here, we describe an effective and unprecedented method for detecting NDV strains of all pathotypes. A conserved region of the fusion protein gene was used for designing oligonucleotides specific to all NDV pathotypes. The dynamic range of this NDV NASBA detection method is comparable to virus culture and therefore the NDV NASBA method is a potential alternative for NDV screening and surveillance.",

keywords = "Disease screening, NASBA, NDV, Newcastle disease, RNA amplicons, Surveillance",

author = "Cui, {Shang Jin} and Fung, {Yin Wan Wendy} and Lau, {Lok Ting} and Liu, {Wen Bin} and Wang, {Yun Feng} and Tong, {Guang Zhi} and Jianguo Chen and Yu, {Albert Cheung Hoi}",

note = "The study was supported by the National Natural Science Foundation of China (grant number: 30170041). The study was supported in part by Chinese Ministry of Agriculture special item Publisher Copyright: {\textcopyright} 2005 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.",

year = "2007",

month = mar,

doi = "10.1016/j.biologicals.2005.12.002",

language = "English",

volume = "35",

pages = "13--18",

journal = "Biologicals",

issn = "1045-1056",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Detection of Newcastle disease virus using nucleic acid sequence-based amplification

AU - Cui, Shang Jin

AU - Fung, Yin Wan Wendy

AU - Lau, Lok Ting

AU - Liu, Wen Bin

AU - Wang, Yun Feng

AU - Tong, Guang Zhi

AU - Chen, Jianguo

AU - Yu, Albert Cheung Hoi

N1 - The study was supported by the National Natural Science Foundation of China (grant number: 30170041). The study was supported in part by Chinese Ministry of Agriculture special item Publisher Copyright: © 2005 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.

PY - 2007/3

Y1 - 2007/3

N2 - Newcastle disease (ND) is a contagious and widespread avian disease affecting most species of birds. ND virus (NDV) is the only member of the avian paramyxovirus serotype 1 (APMV1) causing ND outbreak in bird flocks. The technique of nucleic acid sequence-based amplification (NASBA) is a potential method to rapidly and reliably detect NDV isolates. Here, we describe an effective and unprecedented method for detecting NDV strains of all pathotypes. A conserved region of the fusion protein gene was used for designing oligonucleotides specific to all NDV pathotypes. The dynamic range of this NDV NASBA detection method is comparable to virus culture and therefore the NDV NASBA method is a potential alternative for NDV screening and surveillance.

AB - Newcastle disease (ND) is a contagious and widespread avian disease affecting most species of birds. ND virus (NDV) is the only member of the avian paramyxovirus serotype 1 (APMV1) causing ND outbreak in bird flocks. The technique of nucleic acid sequence-based amplification (NASBA) is a potential method to rapidly and reliably detect NDV isolates. Here, we describe an effective and unprecedented method for detecting NDV strains of all pathotypes. A conserved region of the fusion protein gene was used for designing oligonucleotides specific to all NDV pathotypes. The dynamic range of this NDV NASBA detection method is comparable to virus culture and therefore the NDV NASBA method is a potential alternative for NDV screening and surveillance.

KW - Disease screening

KW - NASBA

KW - NDV

KW - Newcastle disease

KW - RNA amplicons

KW - Surveillance

UR - http://www.scopus.com/inward/record.url?scp=33847119028&partnerID=8YFLogxK

UR - https://www.sciencedirect.com/science/article/pii/S104510560500134X?via%3Dihub

U2 - 10.1016/j.biologicals.2005.12.002

DO - 10.1016/j.biologicals.2005.12.002

M3 - Journal article

C2 - 16500117

AN - SCOPUS:33847119028

SN - 1045-1056

VL - 35

SP - 13

EP - 18

JO - Biologicals

JF - Biologicals

IS - 1

ER -

Detection of Newcastle disease virus using nucleic acid sequence-based amplification

Abstract

Scopus Subject Areas

User-Defined Keywords

UN SDGs

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