Detection of highly pathogenic and low pathogenic avian influenza subtype H5 (Eurasian lineage) using NASBA

Richard A. Collins; Lung Sang Ko; Ka-Lun So; Trevor Ellis; Lok Ting Lau; Albert Cheung Hoi Yu

doi:10.1016/S0166-0934(02)00034-4

Detection of highly pathogenic and low pathogenic avian influenza subtype H5 (Eurasian lineage) using NASBA

Richard A. Collins, Lung Sang Ko, Ka-Lun So, Trevor Ellis, Lok Ting Lau, Albert Cheung Hoi Yu^*

^*Corresponding author for this work

Office of the President and Vice-Chancellor

Research output: Contribution to journal › Journal article › peer-review

60 Citations (Scopus)

Abstract

Nucleic acid sequence-based amplification (NASBA) is a technique that allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A NASBA technique has been developed that allows the detection of avian influenza A subtype H5 from allantoic fluid harvested from inoculated chick embryos. The amplified viral RNA is detected by electrochemiluminescence. The NASBA technique described below is rapid and specific for the identification of influenza A subtype H5 viruses of the Eurasian lineage. More importantly, it can be used to distinguish highly pathogenic and low pathogenic strains of the H5 subtype.

Original language	English
Pages (from-to)	213-225
Number of pages	13
Journal	Journal of Virological Methods
Volume	103
Issue number	2
DOIs	https://doi.org/10.1016/S0166-0934(02)00034-4
Publication status	Published - 16 May 2002

Scopus Subject Areas

Virology

User-Defined Keywords

Avian influenza
Electrochemiluminescence
Eurasian
H5
Haemagglutinin
NASBA
Pathogenic

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/S0166-0934(02)00034-4

Cite this

@article{318b4899089a4134898e716f35f21f3f,

title = "Detection of highly pathogenic and low pathogenic avian influenza subtype H5 (Eurasian lineage) using NASBA",

abstract = "Nucleic acid sequence-based amplification (NASBA) is a technique that allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A NASBA technique has been developed that allows the detection of avian influenza A subtype H5 from allantoic fluid harvested from inoculated chick embryos. The amplified viral RNA is detected by electrochemiluminescence. The NASBA technique described below is rapid and specific for the identification of influenza A subtype H5 viruses of the Eurasian lineage. More importantly, it can be used to distinguish highly pathogenic and low pathogenic strains of the H5 subtype.",

keywords = "Avian influenza, Electrochemiluminescence, Eurasian, H5, Haemagglutinin, NASBA, Pathogenic",

author = "Collins, {Richard A.} and Ko, {Lung Sang} and Ka-Lun So and Trevor Ellis and Lau, {Lok Ting} and Yu, {Albert Cheung Hoi}",

year = "2002",

month = may,

day = "16",

doi = "10.1016/S0166-0934(02)00034-4",

language = "English",

volume = "103",

pages = "213--225",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Detection of highly pathogenic and low pathogenic avian influenza subtype H5 (Eurasian lineage) using NASBA

AU - Collins, Richard A.

AU - Ko, Lung Sang

AU - So, Ka-Lun

AU - Ellis, Trevor

AU - Lau, Lok Ting

AU - Yu, Albert Cheung Hoi

PY - 2002/5/16

Y1 - 2002/5/16

N2 - Nucleic acid sequence-based amplification (NASBA) is a technique that allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A NASBA technique has been developed that allows the detection of avian influenza A subtype H5 from allantoic fluid harvested from inoculated chick embryos. The amplified viral RNA is detected by electrochemiluminescence. The NASBA technique described below is rapid and specific for the identification of influenza A subtype H5 viruses of the Eurasian lineage. More importantly, it can be used to distinguish highly pathogenic and low pathogenic strains of the H5 subtype.

AB - Nucleic acid sequence-based amplification (NASBA) is a technique that allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A NASBA technique has been developed that allows the detection of avian influenza A subtype H5 from allantoic fluid harvested from inoculated chick embryos. The amplified viral RNA is detected by electrochemiluminescence. The NASBA technique described below is rapid and specific for the identification of influenza A subtype H5 viruses of the Eurasian lineage. More importantly, it can be used to distinguish highly pathogenic and low pathogenic strains of the H5 subtype.

KW - Avian influenza

KW - Electrochemiluminescence

KW - Eurasian

KW - H5

KW - Haemagglutinin

KW - NASBA

KW - Pathogenic

UR - http://www.scopus.com/inward/record.url?scp=0037118212&partnerID=8YFLogxK

U2 - 10.1016/S0166-0934(02)00034-4

DO - 10.1016/S0166-0934(02)00034-4

M3 - Journal article

C2 - 12008015

AN - SCOPUS:0037118212

SN - 0166-0934

VL - 103

SP - 213

EP - 225

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 2

ER -

Detection of highly pathogenic and low pathogenic avian influenza subtype H5 (Eurasian lineage) using NASBA

Abstract

Scopus Subject Areas

User-Defined Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this