TY - JOUR
T1 - Detection of foot-and-mouth disease virus by nucleic acid sequence-based amplification (NASBA)
AU - Lau, Lok Ting
AU - Reid, Scott M.
AU - King, Donald P.
AU - Lau, Anson Ming Fung
AU - Shaw, Andrew E.
AU - Ferris, Nigel P.
AU - Yu, Albert Cheung Hoi
N1 - This work was supported in part by Defra project SE1121
Publisher Copyright:
© 2007 Elsevier B.V. All rights reserved.
PY - 2008/1/1
Y1 - 2008/1/1
N2 - A study was conducted to evaluate the performance of a nucleic acid sequence-based amplification (NASBA) assay for the detection of foot-and-mouth disease virus (FMDV). Two detection methods: NASBA-electrochemiluminescence (NASBA-ECL) and a newly developed NASBA-enzyme-linked oligonucleotide capture (NASBA-EOC) were evaluated. The diagnostic sensitivity of these assays was compared with other laboratory-based methods using 200 clinical samples collected from different regions of the world. Assay specificity was also assessed using samples (n = 43) of other viruses that cause vesicular disease in livestock and genetic relatives of FMDV. Concordant results were generated for 174/200 (87.0%) of suspect FMD samples between NASBA-ECL and real-time RT-PCR. In comparison with the virus isolation (VI) data, the sensitivity of the NASBA-ECL assay was 92.9%, which was almost identical to that of the real-time RT-PCR (92.4%) for the same set of samples. There was broad agreement between the results of the NASBA-ECL and the simpler NASBA-EOC detection method for 97.1% of samples. In conclusion, this study provides further data to support the use of NASBA as a rapid and sensitive diagnostic method for the detection and surveillance of FMDV.
AB - A study was conducted to evaluate the performance of a nucleic acid sequence-based amplification (NASBA) assay for the detection of foot-and-mouth disease virus (FMDV). Two detection methods: NASBA-electrochemiluminescence (NASBA-ECL) and a newly developed NASBA-enzyme-linked oligonucleotide capture (NASBA-EOC) were evaluated. The diagnostic sensitivity of these assays was compared with other laboratory-based methods using 200 clinical samples collected from different regions of the world. Assay specificity was also assessed using samples (n = 43) of other viruses that cause vesicular disease in livestock and genetic relatives of FMDV. Concordant results were generated for 174/200 (87.0%) of suspect FMD samples between NASBA-ECL and real-time RT-PCR. In comparison with the virus isolation (VI) data, the sensitivity of the NASBA-ECL assay was 92.9%, which was almost identical to that of the real-time RT-PCR (92.4%) for the same set of samples. There was broad agreement between the results of the NASBA-ECL and the simpler NASBA-EOC detection method for 97.1% of samples. In conclusion, this study provides further data to support the use of NASBA as a rapid and sensitive diagnostic method for the detection and surveillance of FMDV.
KW - Diagnosis
KW - Foot-and-mouth disease virus
KW - NASBA-electrochemiluminescence
KW - NASBA-enzyme-linked oligonucleotide capture
KW - Real-time RT-PCR
UR - http://www.scopus.com/inward/record.url?scp=36549038537&partnerID=8YFLogxK
UR - https://www.sciencedirect.com/science/article/pii/S0378113507003355?via%3Dihub
U2 - 10.1016/j.vetmic.2007.07.008
DO - 10.1016/j.vetmic.2007.07.008
M3 - Journal article
C2 - 17728080
AN - SCOPUS:36549038537
SN - 0378-1135
VL - 126
SP - 101
EP - 110
JO - Veterinary Microbiology
JF - Veterinary Microbiology
IS - 1-3
ER -
