Detection of foot-and-mouth disease virus by nucleic acid sequence-based amplification (NASBA)

Lok Ting Lau; Scott M. Reid; Donald P. King; Anson Ming Fung Lau; Andrew E. Shaw; Nigel P. Ferris; Albert Cheung Hoi Yu

doi:10.1016/j.vetmic.2007.07.008

Detection of foot-and-mouth disease virus by nucleic acid sequence-based amplification (NASBA)

Lok Ting Lau, Scott M. Reid, Donald P. King, Anson Ming Fung Lau, Andrew E. Shaw, Nigel P. Ferris, Albert Cheung Hoi Yu^*

^*Corresponding author for this work

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Abstract

A study was conducted to evaluate the performance of a nucleic acid sequence-based amplification (NASBA) assay for the detection of foot-and-mouth disease virus (FMDV). Two detection methods: NASBA-electrochemiluminescence (NASBA-ECL) and a newly developed NASBA-enzyme-linked oligonucleotide capture (NASBA-EOC) were evaluated. The diagnostic sensitivity of these assays was compared with other laboratory-based methods using 200 clinical samples collected from different regions of the world. Assay specificity was also assessed using samples (n = 43) of other viruses that cause vesicular disease in livestock and genetic relatives of FMDV. Concordant results were generated for 174/200 (87.0%) of suspect FMD samples between NASBA-ECL and real-time RT-PCR. In comparison with the virus isolation (VI) data, the sensitivity of the NASBA-ECL assay was 92.9%, which was almost identical to that of the real-time RT-PCR (92.4%) for the same set of samples. There was broad agreement between the results of the NASBA-ECL and the simpler NASBA-EOC detection method for 97.1% of samples. In conclusion, this study provides further data to support the use of NASBA as a rapid and sensitive diagnostic method for the detection and surveillance of FMDV.

Original language	English
Pages (from-to)	101-110
Number of pages	10
Journal	Veterinary Microbiology
Volume	126
Issue number	1-3
DOIs	https://doi.org/10.1016/j.vetmic.2007.07.008
Publication status	Published - 1 Jan 2008

User-Defined Keywords

Diagnosis
Foot-and-mouth disease virus
NASBA-electrochemiluminescence
NASBA-enzyme-linked oligonucleotide capture
Real-time RT-PCR

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vetmic.2007.07.008

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@article{19ce18b2cb0a4c49882b8c93a98a3ce5,

title = "Detection of foot-and-mouth disease virus by nucleic acid sequence-based amplification (NASBA)",

abstract = "A study was conducted to evaluate the performance of a nucleic acid sequence-based amplification (NASBA) assay for the detection of foot-and-mouth disease virus (FMDV). Two detection methods: NASBA-electrochemiluminescence (NASBA-ECL) and a newly developed NASBA-enzyme-linked oligonucleotide capture (NASBA-EOC) were evaluated. The diagnostic sensitivity of these assays was compared with other laboratory-based methods using 200 clinical samples collected from different regions of the world. Assay specificity was also assessed using samples (n = 43) of other viruses that cause vesicular disease in livestock and genetic relatives of FMDV. Concordant results were generated for 174/200 (87.0%) of suspect FMD samples between NASBA-ECL and real-time RT-PCR. In comparison with the virus isolation (VI) data, the sensitivity of the NASBA-ECL assay was 92.9%, which was almost identical to that of the real-time RT-PCR (92.4%) for the same set of samples. There was broad agreement between the results of the NASBA-ECL and the simpler NASBA-EOC detection method for 97.1% of samples. In conclusion, this study provides further data to support the use of NASBA as a rapid and sensitive diagnostic method for the detection and surveillance of FMDV.",

keywords = "Diagnosis, Foot-and-mouth disease virus, NASBA-electrochemiluminescence, NASBA-enzyme-linked oligonucleotide capture, Real-time RT-PCR",

author = "Lau, {Lok Ting} and Reid, {Scott M.} and King, {Donald P.} and Lau, {Anson Ming Fung} and Shaw, {Andrew E.} and Ferris, {Nigel P.} and Yu, {Albert Cheung Hoi}",

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T1 - Detection of foot-and-mouth disease virus by nucleic acid sequence-based amplification (NASBA)

AU - Lau, Lok Ting

AU - Reid, Scott M.

AU - King, Donald P.

AU - Lau, Anson Ming Fung

AU - Shaw, Andrew E.

AU - Ferris, Nigel P.

AU - Yu, Albert Cheung Hoi

PY - 2008/1/1

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N2 - A study was conducted to evaluate the performance of a nucleic acid sequence-based amplification (NASBA) assay for the detection of foot-and-mouth disease virus (FMDV). Two detection methods: NASBA-electrochemiluminescence (NASBA-ECL) and a newly developed NASBA-enzyme-linked oligonucleotide capture (NASBA-EOC) were evaluated. The diagnostic sensitivity of these assays was compared with other laboratory-based methods using 200 clinical samples collected from different regions of the world. Assay specificity was also assessed using samples (n = 43) of other viruses that cause vesicular disease in livestock and genetic relatives of FMDV. Concordant results were generated for 174/200 (87.0%) of suspect FMD samples between NASBA-ECL and real-time RT-PCR. In comparison with the virus isolation (VI) data, the sensitivity of the NASBA-ECL assay was 92.9%, which was almost identical to that of the real-time RT-PCR (92.4%) for the same set of samples. There was broad agreement between the results of the NASBA-ECL and the simpler NASBA-EOC detection method for 97.1% of samples. In conclusion, this study provides further data to support the use of NASBA as a rapid and sensitive diagnostic method for the detection and surveillance of FMDV.

AB - A study was conducted to evaluate the performance of a nucleic acid sequence-based amplification (NASBA) assay for the detection of foot-and-mouth disease virus (FMDV). Two detection methods: NASBA-electrochemiluminescence (NASBA-ECL) and a newly developed NASBA-enzyme-linked oligonucleotide capture (NASBA-EOC) were evaluated. The diagnostic sensitivity of these assays was compared with other laboratory-based methods using 200 clinical samples collected from different regions of the world. Assay specificity was also assessed using samples (n = 43) of other viruses that cause vesicular disease in livestock and genetic relatives of FMDV. Concordant results were generated for 174/200 (87.0%) of suspect FMD samples between NASBA-ECL and real-time RT-PCR. In comparison with the virus isolation (VI) data, the sensitivity of the NASBA-ECL assay was 92.9%, which was almost identical to that of the real-time RT-PCR (92.4%) for the same set of samples. There was broad agreement between the results of the NASBA-ECL and the simpler NASBA-EOC detection method for 97.1% of samples. In conclusion, this study provides further data to support the use of NASBA as a rapid and sensitive diagnostic method for the detection and surveillance of FMDV.

KW - Diagnosis

KW - Foot-and-mouth disease virus

KW - NASBA-electrochemiluminescence

KW - NASBA-enzyme-linked oligonucleotide capture

KW - Real-time RT-PCR

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JF - Veterinary Microbiology

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ER -

Detection of foot-and-mouth disease virus by nucleic acid sequence-based amplification (NASBA)

Abstract

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