Detection of ethanol in food: A new biosensor based on bacteria

Guangming Wen; Zhongping Li; Martin M F CHOI

doi:10.1016/j.jfoodeng.2013.01.006

Detection of ethanol in food: A new biosensor based on bacteria

Guangming Wen^*, Zhongping Li, Martin M F CHOI

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

24 Citations (Scopus)

Abstract

A microbial biosensor for determination of ethanol has been developed. The microbial ethanol biosensor comprises a Methylobacterium organophilium-immobilized eggshell membrane and an oxygen (O₂) electrode. The microbial biosensor responds linearly to ethanol in the range 0.050–7.5 mM with a detection limit of 0.025 mM (S/N = 3) and the response time is 100 s. The optimal loading of bacterial cells on the biosensor membrane is 40 mg (wet weight). The optimal working conditions for the microbial biosensor are pH 7.0 phosphate buffer (50 mM) at 20–25 °C. The interference test, operational and storage stability of the biosensor are studied in detail. Finally, the biosensor is applied to determine the ethanol contents in various alcohol samples and the results are comparable to that obtained by a gas chromatographic method. Our work demonstrates that the proposed microbial biosensor is a reliable method to determine the ethanol content in wine samples.

Original language	English
Pages (from-to)	56-61
Number of pages	6
Journal	Journal of Food Engineering
Volume	118
Issue number	1
DOIs	https://doi.org/10.1016/j.jfoodeng.2013.01.006
Publication status	Published - 1 Sept 2013

Scopus Subject Areas

Food Science

User-Defined Keywords

Eggshell membrane
Ethanol
Methylobacterium organophilium
Microbial biosensor
Oxygen electrode

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10.1016/j.jfoodeng.2013.01.006

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@article{f6884680600d40e095e1d66d984279ce,

title = "Detection of ethanol in food: A new biosensor based on bacteria",

abstract = "A microbial biosensor for determination of ethanol has been developed. The microbial ethanol biosensor comprises a Methylobacterium organophilium-immobilized eggshell membrane and an oxygen (O2) electrode. The microbial biosensor responds linearly to ethanol in the range 0.050–7.5 mM with a detection limit of 0.025 mM (S/N = 3) and the response time is 100 s. The optimal loading of bacterial cells on the biosensor membrane is 40 mg (wet weight). The optimal working conditions for the microbial biosensor are pH 7.0 phosphate buffer (50 mM) at 20–25 °C. The interference test, operational and storage stability of the biosensor are studied in detail. Finally, the biosensor is applied to determine the ethanol contents in various alcohol samples and the results are comparable to that obtained by a gas chromatographic method. Our work demonstrates that the proposed microbial biosensor is a reliable method to determine the ethanol content in wine samples.",

keywords = "Eggshell membrane, Ethanol, Methylobacterium organophilium, Microbial biosensor, Oxygen electrode",

author = "Guangming Wen and Zhongping Li and CHOI, {Martin M F}",

note = "Funding Information: The work described in this article was supported by the Hundred Talent Programme of Shanxi Province, Youth Science Foundation of Shanxi Province (2010021014), Doctoral United Fund of Education Ministry (20111401120003), and National Natural Science Foundation of China (21071092).",

year = "2013",

month = sep,

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doi = "10.1016/j.jfoodeng.2013.01.006",

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T1 - Detection of ethanol in food

T2 - A new biosensor based on bacteria

AU - Wen, Guangming

AU - Li, Zhongping

AU - CHOI, Martin M F

N1 - Funding Information: The work described in this article was supported by the Hundred Talent Programme of Shanxi Province, Youth Science Foundation of Shanxi Province (2010021014), Doctoral United Fund of Education Ministry (20111401120003), and National Natural Science Foundation of China (21071092).

PY - 2013/9/1

Y1 - 2013/9/1

N2 - A microbial biosensor for determination of ethanol has been developed. The microbial ethanol biosensor comprises a Methylobacterium organophilium-immobilized eggshell membrane and an oxygen (O2) electrode. The microbial biosensor responds linearly to ethanol in the range 0.050–7.5 mM with a detection limit of 0.025 mM (S/N = 3) and the response time is 100 s. The optimal loading of bacterial cells on the biosensor membrane is 40 mg (wet weight). The optimal working conditions for the microbial biosensor are pH 7.0 phosphate buffer (50 mM) at 20–25 °C. The interference test, operational and storage stability of the biosensor are studied in detail. Finally, the biosensor is applied to determine the ethanol contents in various alcohol samples and the results are comparable to that obtained by a gas chromatographic method. Our work demonstrates that the proposed microbial biosensor is a reliable method to determine the ethanol content in wine samples.

AB - A microbial biosensor for determination of ethanol has been developed. The microbial ethanol biosensor comprises a Methylobacterium organophilium-immobilized eggshell membrane and an oxygen (O2) electrode. The microbial biosensor responds linearly to ethanol in the range 0.050–7.5 mM with a detection limit of 0.025 mM (S/N = 3) and the response time is 100 s. The optimal loading of bacterial cells on the biosensor membrane is 40 mg (wet weight). The optimal working conditions for the microbial biosensor are pH 7.0 phosphate buffer (50 mM) at 20–25 °C. The interference test, operational and storage stability of the biosensor are studied in detail. Finally, the biosensor is applied to determine the ethanol contents in various alcohol samples and the results are comparable to that obtained by a gas chromatographic method. Our work demonstrates that the proposed microbial biosensor is a reliable method to determine the ethanol content in wine samples.

KW - Eggshell membrane

KW - Ethanol

KW - Methylobacterium organophilium

KW - Microbial biosensor

KW - Oxygen electrode

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DO - 10.1016/j.jfoodeng.2013.01.006

M3 - Journal article

AN - SCOPUS:84876664890

SN - 0260-8774

VL - 118

SP - 56

EP - 61

JO - Journal of Food Engineering

JF - Journal of Food Engineering

IS - 1

ER -

Detection of ethanol in food: A new biosensor based on bacteria

Abstract

Scopus Subject Areas

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