Detection of base excision repair enzyme activity using a luminescent g-quadruplex selective switch-on probe

Ka Ho Leung; Hong Zhang He; Victor Pui Yan Ma; Hai Jing Zhong; Daniel Shiu Hin Chan; Jun Zhou; Jean Louis Mergny; Chung Hang Leung; Dik Lung Ma

doi:10.1039/c3cc41129j

Detection of base excision repair enzyme activity using a luminescent g-quadruplex selective switch-on probe

Ka Ho Leung
, Hong Zhang He
, Victor Pui Yan Ma
, Hai Jing Zhong
, Daniel Shiu Hin Chan
, Jun Zhou
, Jean Louis Mergny
, Chung Hang Leung
, Dik Lung Ma^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

115 Citations (Scopus)

Abstract

We report herein a simple and convenient luminescent assay for detection of base excision repair enzyme activity using an Ir(III) complex as a G-quadruplex selective probe. Using uracil-DNA glycosylase (UDG) as a model enzyme, the assay achieved high sensitivity and selectivity for UDG over other tested enzymes. The utility of the assay for screening potential UDG inhibitors was also demonstrated.

Original language	English
Pages (from-to)	5630-5632
Number of pages	3
Journal	Chemical Communications
Volume	49
Issue number	50
DOIs	https://doi.org/10.1039/c3cc41129j
Publication status	Published - 28 May 2013

Access to Document

10.1039/c3cc41129j

Cite this

@article{6c0cb28152b14e81b49843515443d617,

title = "Detection of base excision repair enzyme activity using a luminescent g-quadruplex selective switch-on probe",

abstract = "We report herein a simple and convenient luminescent assay for detection of base excision repair enzyme activity using an Ir(III) complex as a G-quadruplex selective probe. Using uracil-DNA glycosylase (UDG) as a model enzyme, the assay achieved high sensitivity and selectivity for UDG over other tested enzymes. The utility of the assay for screening potential UDG inhibitors was also demonstrated.",

author = "Leung, \{Ka Ho\} and He, \{Hong Zhang\} and Ma, \{Victor Pui Yan\} and Zhong, \{Hai Jing\} and Chan, \{Daniel Shiu Hin\} and Jun Zhou and Mergny, \{Jean Louis\} and Leung, \{Chung Hang\} and Ma, \{Dik Lung\}",

note = "This work was supported by Hong Kong Baptist University (FRG2/11-12/009), Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/11101212), the Research Grants Council (HKBU/201811 and HKBU/204612), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12), the Science and Technology Development Fund, Macao SAR (001/2012/A) and the University of Macau (MYRG091(Y2-L2)-ICMS12-LCH and MYRG121(Y2-L2)-ICMS12-LCH).",

year = "2013",

month = may,

day = "28",

doi = "10.1039/c3cc41129j",

language = "English",

volume = "49",

pages = "5630--5632",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "50",

}

TY - JOUR

T1 - Detection of base excision repair enzyme activity using a luminescent g-quadruplex selective switch-on probe

AU - Leung, Ka Ho

AU - He, Hong Zhang

AU - Ma, Victor Pui Yan

AU - Zhong, Hai Jing

AU - Chan, Daniel Shiu Hin

AU - Zhou, Jun

AU - Mergny, Jean Louis

AU - Leung, Chung Hang

AU - Ma, Dik Lung

N1 - This work was supported by Hong Kong Baptist University (FRG2/11-12/009), Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/11101212), the Research Grants Council (HKBU/201811 and HKBU/204612), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12), the Science and Technology Development Fund, Macao SAR (001/2012/A) and the University of Macau (MYRG091(Y2-L2)-ICMS12-LCH and MYRG121(Y2-L2)-ICMS12-LCH).

PY - 2013/5/28

Y1 - 2013/5/28

N2 - We report herein a simple and convenient luminescent assay for detection of base excision repair enzyme activity using an Ir(III) complex as a G-quadruplex selective probe. Using uracil-DNA glycosylase (UDG) as a model enzyme, the assay achieved high sensitivity and selectivity for UDG over other tested enzymes. The utility of the assay for screening potential UDG inhibitors was also demonstrated.

AB - We report herein a simple and convenient luminescent assay for detection of base excision repair enzyme activity using an Ir(III) complex as a G-quadruplex selective probe. Using uracil-DNA glycosylase (UDG) as a model enzyme, the assay achieved high sensitivity and selectivity for UDG over other tested enzymes. The utility of the assay for screening potential UDG inhibitors was also demonstrated.

UR - https://www.scopus.com/pages/publications/84878659357

U2 - 10.1039/c3cc41129j

DO - 10.1039/c3cc41129j

M3 - Journal article

C2 - 23559154

AN - SCOPUS:84878659357

SN - 1359-7345

VL - 49

SP - 5630

EP - 5632

JO - Chemical Communications

JF - Chemical Communications

IS - 50

ER -

Detection of base excision repair enzyme activity using a luminescent g-quadruplex selective switch-on probe

Abstract

Access to Document

Other files and links

Fingerprint

Cite this