Detection of base excision repair enzyme activity using a luminescent g-quadruplex selective switch-on probe

Ka Ho Leung, Hong Zhang He, Victor Pui Yan Ma, Hai Jing Zhong, Daniel Shiu Hin Chan, Jun Zhou, Jean Louis Mergny, Chung Hang Leung, Edmond Dik Lung MA*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

105 Citations (Scopus)

Abstract

We report herein a simple and convenient luminescent assay for detection of base excision repair enzyme activity using an Ir(III) complex as a G-quadruplex selective probe. Using uracil-DNA glycosylase (UDG) as a model enzyme, the assay achieved high sensitivity and selectivity for UDG over other tested enzymes. The utility of the assay for screening potential UDG inhibitors was also demonstrated.

Original languageEnglish
Pages (from-to)5630-5632
Number of pages3
JournalChemical Communications
Volume49
Issue number50
DOIs
Publication statusPublished - 28 May 2013

Scopus Subject Areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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