Destiny of Dendrobium officinale Polysaccharide after Oral Administration: Indigestible and Nonabsorbing, Ends in Modulating Gut Microbiota

Lifeng Li; Hong Yao; Xiaojun Li; Quanwei Zhang; Xiyang Wu; Tinlong Wong; Hongming Zheng; Hauyee Fung; Bingxun Yang; Diklung Ma; Chunghang Leung; Ge Zhang; Zhaoxiang Bian; Aiping Lu; Quanbin Han

doi:10.1021/acs.jafc.9b01489

Destiny of Dendrobium officinale Polysaccharide after Oral Administration: Indigestible and Nonabsorbing, Ends in Modulating Gut Microbiota

Lifeng Li, Hong Yao, Xiaojun Li, Quanwei Zhang, Xiyang Wu^*, Tinlong Wong, Hongming Zheng, Hauyee Fung, Bingxun Yang, Diklung Ma, Chunghang Leung, Ge Zhang, Zhaoxiang Bian, Aiping Lu, Quanbin Han^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

121 Citations (Scopus)

Abstract

Polysaccharides from functional foods have been proved to have diverse bioactivities, but little is known about what exactly happens to these polysaccharides after oral administration and even less about the underlying mechanism of action. Taking the marker polysaccharide (DOP) of Dendrobium officinale as an example, this study aims to demonstrate the dynamic distribution and degradation of orally dosed DOP in mice and in vitro using near-infrared fluorescence imaging and a kind of chromatographic analysis. The results indicate that (1) neither DOP nor fluorescence-labeled DOP (FDOP) was absorbed, (2) both DOP and FDOP were undigested and were quickly degraded to short-chain fatty acids in the large intestine, (3) DOP modulated gut microbiota, which could be associated with DOP's suppression of 4T1 tumor growth in mice. All of these findings suggest that some (maybe not all) bioactive polysaccharides share a common destiny: indigestible and nonabsorbing, ends in modulating bioactivities-associated gut microbiota.

Original language	English
Pages (from-to)	5968-5977
Number of pages	10
Journal	Journal of Agricultural and Food Chemistry
Volume	67
Issue number	21
Early online date	30 Apr 2019
DOIs	https://doi.org/10.1021/acs.jafc.9b01489
Publication status	Published - 29 May 2019

Scopus Subject Areas

Chemistry(all)
Agricultural and Biological Sciences(all)

User-Defined Keywords

absorption
antitumor
Dendrobium officinale
digestion
gut microbiota
polysaccharide

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10.1021/acs.jafc.9b01489

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Li, L., Yao, H., Li, X., Zhang, Q., Wu, X., Wong, T., Zheng, H., Fung, H., Yang, B., Ma, D., Leung, C., Zhang, G., Bian, Z., Lu, A., & Han, Q. (2019). Destiny of Dendrobium officinale Polysaccharide after Oral Administration: Indigestible and Nonabsorbing, Ends in Modulating Gut Microbiota. Journal of Agricultural and Food Chemistry, 67(21), 5968-5977. https://doi.org/10.1021/acs.jafc.9b01489

@article{151ce9e7591f436aabcc93583084fb83,

title = "Destiny of Dendrobium officinale Polysaccharide after Oral Administration: Indigestible and Nonabsorbing, Ends in Modulating Gut Microbiota",

abstract = "Polysaccharides from functional foods have been proved to have diverse bioactivities, but little is known about what exactly happens to these polysaccharides after oral administration and even less about the underlying mechanism of action. Taking the marker polysaccharide (DOP) of Dendrobium officinale as an example, this study aims to demonstrate the dynamic distribution and degradation of orally dosed DOP in mice and in vitro using near-infrared fluorescence imaging and a kind of chromatographic analysis. The results indicate that (1) neither DOP nor fluorescence-labeled DOP (FDOP) was absorbed, (2) both DOP and FDOP were undigested and were quickly degraded to short-chain fatty acids in the large intestine, (3) DOP modulated gut microbiota, which could be associated with DOP's suppression of 4T1 tumor growth in mice. All of these findings suggest that some (maybe not all) bioactive polysaccharides share a common destiny: indigestible and nonabsorbing, ends in modulating bioactivities-associated gut microbiota.",

keywords = "absorption, antitumor, Dendrobium officinale, digestion, gut microbiota, polysaccharide",

author = "Lifeng Li and Hong Yao and Xiaojun Li and Quanwei Zhang and Xiyang Wu and Tinlong Wong and Hongming Zheng and Hauyee Fung and Bingxun Yang and Diklung Ma and Chunghang Leung and Ge Zhang and Zhaoxiang Bian and Aiping Lu and Quanbin Han",

note = "Funding information: This study was supported by the HKSAR Innovation and Technology Fund (ITF), Tier 3, ITS/311/09, General Research Fund (12100615, 22100014, 12100818), Health Medical Research Fund (11122531, 14150521), National Natural Sciences Foundation in China (81473341), and Hong Kong Baptist University (RC-start up grant, MPCF-001–2014/2015, RC-IRMS/14–15/06, FRG2/17–18/060, and FRG2/16–17/002). Publisher copyright: {\textcopyright} 2019 American Chemical Society",

year = "2019",

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doi = "10.1021/acs.jafc.9b01489",

language = "English",

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Li, L, Yao, H, Li, X, Zhang, Q, Wu, X, Wong, T, Zheng, H, Fung, H, Yang, B, Ma, D, Leung, C, Zhang, G , Bian, Z , Lu, A & Han, Q 2019, 'Destiny of Dendrobium officinale Polysaccharide after Oral Administration: Indigestible and Nonabsorbing, Ends in Modulating Gut Microbiota', Journal of Agricultural and Food Chemistry, vol. 67, no. 21, pp. 5968-5977. https://doi.org/10.1021/acs.jafc.9b01489

TY - JOUR

T1 - Destiny of Dendrobium officinale Polysaccharide after Oral Administration

T2 - Indigestible and Nonabsorbing, Ends in Modulating Gut Microbiota

AU - Li, Lifeng

AU - Yao, Hong

AU - Li, Xiaojun

AU - Zhang, Quanwei

AU - Wu, Xiyang

AU - Wong, Tinlong

AU - Zheng, Hongming

AU - Fung, Hauyee

AU - Yang, Bingxun

AU - Ma, Diklung

AU - Leung, Chunghang

AU - Zhang, Ge

AU - Bian, Zhaoxiang

AU - Lu, Aiping

AU - Han, Quanbin

N1 - Funding information: This study was supported by the HKSAR Innovation and Technology Fund (ITF), Tier 3, ITS/311/09, General Research Fund (12100615, 22100014, 12100818), Health Medical Research Fund (11122531, 14150521), National Natural Sciences Foundation in China (81473341), and Hong Kong Baptist University (RC-start up grant, MPCF-001–2014/2015, RC-IRMS/14–15/06, FRG2/17–18/060, and FRG2/16–17/002). Publisher copyright: © 2019 American Chemical Society

PY - 2019/5/29

Y1 - 2019/5/29

N2 - Polysaccharides from functional foods have been proved to have diverse bioactivities, but little is known about what exactly happens to these polysaccharides after oral administration and even less about the underlying mechanism of action. Taking the marker polysaccharide (DOP) of Dendrobium officinale as an example, this study aims to demonstrate the dynamic distribution and degradation of orally dosed DOP in mice and in vitro using near-infrared fluorescence imaging and a kind of chromatographic analysis. The results indicate that (1) neither DOP nor fluorescence-labeled DOP (FDOP) was absorbed, (2) both DOP and FDOP were undigested and were quickly degraded to short-chain fatty acids in the large intestine, (3) DOP modulated gut microbiota, which could be associated with DOP's suppression of 4T1 tumor growth in mice. All of these findings suggest that some (maybe not all) bioactive polysaccharides share a common destiny: indigestible and nonabsorbing, ends in modulating bioactivities-associated gut microbiota.

AB - Polysaccharides from functional foods have been proved to have diverse bioactivities, but little is known about what exactly happens to these polysaccharides after oral administration and even less about the underlying mechanism of action. Taking the marker polysaccharide (DOP) of Dendrobium officinale as an example, this study aims to demonstrate the dynamic distribution and degradation of orally dosed DOP in mice and in vitro using near-infrared fluorescence imaging and a kind of chromatographic analysis. The results indicate that (1) neither DOP nor fluorescence-labeled DOP (FDOP) was absorbed, (2) both DOP and FDOP were undigested and were quickly degraded to short-chain fatty acids in the large intestine, (3) DOP modulated gut microbiota, which could be associated with DOP's suppression of 4T1 tumor growth in mice. All of these findings suggest that some (maybe not all) bioactive polysaccharides share a common destiny: indigestible and nonabsorbing, ends in modulating bioactivities-associated gut microbiota.

KW - absorption

KW - antitumor

KW - Dendrobium officinale

KW - digestion

KW - gut microbiota

KW - polysaccharide

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U2 - 10.1021/acs.jafc.9b01489

DO - 10.1021/acs.jafc.9b01489

M3 - Journal article

C2 - 31037938

AN - SCOPUS:85066855471

SN - 0021-8561

VL - 67

SP - 5968

EP - 5977

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 21

ER -

Destiny of Dendrobium officinale Polysaccharide after Oral Administration: Indigestible and Nonabsorbing, Ends in Modulating Gut Microbiota

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