Design of ratiometric emission probe with visible light excitation for determination of Ca2+ in living cells

Qiaoling Liu, Huizhi Du, Xiaoze Ren, Wei Bian, Li Fan, Shaomin Shuang, Chuan Dong*, Qin Hu, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

An organic salt as a fluorescent probe based on intramolecular charge transfer for Ca2+ determination is developed. Ca2+ can be detected by ratiometric emission at 490 and 594 nm with an excitation wavelength of 405 nm. This probe is highly selective for Ca2+ over other divalent metal cations and displays a large Stokes shift of 189 nm that can avoid interference of the excitation light beam and autofluorescence of biological samples. The dissociation constant for Ca2+ is 2.25 ± 0.47 μM and pertinent to Ca2+ detection in cellular resting and dynamic states. The probe demonstrates its application in monitoring Ca2+ in living cells under confocal microscopic imaging.

Original languageEnglish
Pages (from-to)8025-8030
Number of pages6
JournalAnalytical Chemistry
Volume86
Issue number16
DOIs
Publication statusPublished - 19 Aug 2014

Scopus Subject Areas

  • Analytical Chemistry

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