Desiccation tolerance in bryophytes: The rehydration proteomes of Bryum argenteum provide insights into the resuscitation mechanism

Bei Gao, Daoyuan Zhang*, Xiaoshuang Li, Honglan Yang, Yuqing Liang, Moxian Chen, Yuanming Zhang, Jianhua ZHANG, Andrew Wood

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

9 Citations (Scopus)


Bryum argenteum Hedw. is a desiccation tolerant bryophyte and belongs to one of the most important components of the biological soil crusts (BSCs) found in the deserts of Central Asia. Limited information is available on rehydration-responsive proteins in desiccation tolerant plants. As a complement to our previous research analyzing the rehydration transcriptome, we present a parallel quantitative proteomic effort to study rehydration-responsive proteins. Bryophyte gametophores were desiccated (Dry) and rehydrated for 2 h (R2) and 24 h (R24). Proteins from Dry, R2 and R24 gametophores were labeled by isobaric tags for relative and absolute quantitation (iTRAQ) to determine the relative abundance of rehydration-responsive proteins. A total of 5503 non-redundant protein sequences were identified and 4772 (86.7%) protein sequences were annotated using Gene Ontology (GO) terms and Pfam classifications. Upon rehydration 239 proteins were elevated and 461 proteins were reduced as compared to the desiccated protein sample. Differentially up-regulated proteins were classified into a number of categories including reactive oxygen species scavenging enzymes, detoxifying enzymes, Late Embryogenesis Abundant (LEA) proteins, heat shock proteins, proteasome components and proteases, and photosynthesis and translation related proteins. Furthermore, the results of the correlation between transcriptome and proteome revealed the discordant changes in the expression between protein and mRNA.

Original languageEnglish
Pages (from-to)152-167
Number of pages16
JournalJournal of Arid Land
Issue number1
Publication statusPublished - Feb 2018

Scopus Subject Areas

  • Water Science and Technology
  • Earth-Surface Processes
  • Management, Monitoring, Policy and Law

User-Defined Keywords

  • Bryum
  • Desiccation tolerance
  • iTRAQ
  • Physcomitrella patens
  • Proteome
  • Rehydration


Dive into the research topics of 'Desiccation tolerance in bryophytes: The rehydration proteomes of Bryum argenteum provide insights into the resuscitation mechanism'. Together they form a unique fingerprint.

Cite this