TY - JOUR
T1 - Derivatization of N-Acyl Glycines by 3-Nitrophenylhydrazine for Targeted Metabolomics Analysis and Their Application to the Study of Diabetes Progression in Mice
AU - Xiang, Li
AU - Ru, Yi
AU - Shi, Jingchun
AU - Wang, Li
AU - Zhao, Hongzhi
AU - Huang, Yu
AU - Cai, Zongwei
N1 - Funding Information:
We are grateful to all the participants in this study. The authors also would like to acknowledge the financial support from the Research Grants Council of Hong Kong Collaborative Research Found Group Research Projects Grant C4024-16 W and Hong Kong Research Grants Council Area of Excellence (AoE/M/707-18).
Publisher copyright:
© 2023 The Authors. Published by American Chemical Society
PY - 2023/1/31
Y1 - 2023/1/31
N2 - N-Acyl glycines (NAGlys) are an important class of metabolites in the
detoxification system of the human body. They have been used in the
diagnosis of several metabolic diseases. Liquid chromatography–mass
spectrometry (LC–MS) is the most frequently used NAGlys detection
platform. Here, we describe a simple and sensitive method of NAGlys
detection by LC–MS in plasma and urine samples. This approach is based
on the use of a derivatization reagent, 3-nitrophenylhydrazine. The
reaction is quick in aqueous solution, and no quenching step is needed.
To expand the coverage of NAGlys when standards are not available,
NAGlys were first identified based on high-resolution LC–MS.
Quantification was subsequently carried out on triple quadrupole LC–MS.
This approach allowed a much broader measurement of NAGlys (41 NAGlys in
total), especially when authentic standards are unavailable.
Comprehensive analysis of NAGlys with this new method was applied in
plasma and urine samples of db/db diabetic and non-diabetic db/m+
control mice. The majority of detected NAGlys were altered with high
differentiation ability in plasma and urine samples from diabetic and
non-diabetic mice. These identified NAGlys hold the potential to be
diagnostic biomarkers for type II diabetes and diabetic complications.
AB - N-Acyl glycines (NAGlys) are an important class of metabolites in the
detoxification system of the human body. They have been used in the
diagnosis of several metabolic diseases. Liquid chromatography–mass
spectrometry (LC–MS) is the most frequently used NAGlys detection
platform. Here, we describe a simple and sensitive method of NAGlys
detection by LC–MS in plasma and urine samples. This approach is based
on the use of a derivatization reagent, 3-nitrophenylhydrazine. The
reaction is quick in aqueous solution, and no quenching step is needed.
To expand the coverage of NAGlys when standards are not available,
NAGlys were first identified based on high-resolution LC–MS.
Quantification was subsequently carried out on triple quadrupole LC–MS.
This approach allowed a much broader measurement of NAGlys (41 NAGlys in
total), especially when authentic standards are unavailable.
Comprehensive analysis of NAGlys with this new method was applied in
plasma and urine samples of db/db diabetic and non-diabetic db/m+
control mice. The majority of detected NAGlys were altered with high
differentiation ability in plasma and urine samples from diabetic and
non-diabetic mice. These identified NAGlys hold the potential to be
diagnostic biomarkers for type II diabetes and diabetic complications.
UR - http://www.scopus.com/inward/record.url?scp=85146543238&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.2c02507
DO - 10.1021/acs.analchem.2c02507
M3 - Journal article
C2 - 36657965
SN - 0003-2700
VL - 95
SP - 2183
EP - 2191
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 4
ER -
