TY - JOUR
T1 - Degradation of hydrocarbons by indigenous microbial communities from two adjacent oil production wells in one block
AU - Liu, Haijun
AU - Yao, Jun
AU - Yuan, Zhimin
AU - Chen, Huilun
AU - Wang, Fei
AU - Masakorala, Kanaji
AU - Choi, Martin M.F.
N1 - Funding Information:
This work is supported in part by grants from the International Joint Key Project from the Chinese Ministry of Science and Technology (2010DFB23160), the National Natural Science Foundation of China (41430106, 41273092, 41103060, 41103058 and 41603083), and Public welfare project of the Chinese Ministry of Environmental Protection (201409042, 201509049), and Key Project Supported by the Natural Science Foundation of the Higher Education Institutions of Anhui Province (KJ2016A432). We gratefully acknowledge all the support provided.
PY - 2016/12/1
Y1 - 2016/12/1
N2 - Aerobic acclimatization cultures of oil production water from two adjacent oil production wells (Xi15-14 and Xi51-5) in one block of Dagang oilfield were generated depending on crude oil as the sole carbon source and energy. Both cultures revealed a high degrading efficiency for a wide range of hydrocarbons, but degradation trend were striking different, and surface tension of two phases of oil and water decreased from 60 to 30 mN/m approximately by virtue of indigenous microbial metabolic activities. Meanwhile, cultured indigenous bacteria of wellXi15-14 mainly included Hydrocarboniphaga, Pseudomonas, and Ectothiorhodospiraceae bacterium, while wellXi51-5 contained Sphingomonas and Pseudomonas. Furthermore, abundance of alkane hydroxylase genes alk B and alk M from the two samples also showed an apparent difference. These findings are important that microbial diversity tightly tallies with the fact of a highly compartmentalized stratigraphy of this oilfield, suggesting that it is better to recover oil from extreme reservoirs with the targeted stimulating indigenous microorganisms.
AB - Aerobic acclimatization cultures of oil production water from two adjacent oil production wells (Xi15-14 and Xi51-5) in one block of Dagang oilfield were generated depending on crude oil as the sole carbon source and energy. Both cultures revealed a high degrading efficiency for a wide range of hydrocarbons, but degradation trend were striking different, and surface tension of two phases of oil and water decreased from 60 to 30 mN/m approximately by virtue of indigenous microbial metabolic activities. Meanwhile, cultured indigenous bacteria of wellXi15-14 mainly included Hydrocarboniphaga, Pseudomonas, and Ectothiorhodospiraceae bacterium, while wellXi51-5 contained Sphingomonas and Pseudomonas. Furthermore, abundance of alkane hydroxylase genes alk B and alk M from the two samples also showed an apparent difference. These findings are important that microbial diversity tightly tallies with the fact of a highly compartmentalized stratigraphy of this oilfield, suggesting that it is better to recover oil from extreme reservoirs with the targeted stimulating indigenous microorganisms.
KW - Degradation
KW - indigenous microorganism
KW - MEOR
KW - microbial diversity
KW - oil production water
UR - http://www.scopus.com/inward/record.url?scp=84992065759&partnerID=8YFLogxK
U2 - 10.1080/15567036.2014.943853
DO - 10.1080/15567036.2014.943853
M3 - Journal article
AN - SCOPUS:84992065759
SN - 1556-7036
VL - 38
SP - 3423
EP - 3434
JO - Energy Sources, Part A: Recovery, Utilization and Environmental Effects
JF - Energy Sources, Part A: Recovery, Utilization and Environmental Effects
IS - 23
ER -
