DeepDRIM: a deep neural network to reconstruct cell-type-specific gene regulatory network using single-cell RNA-Seq Data

Jiaxing Chen, Chinwang Cheong, Liang Lan, Xin Zhou, Jiming Liu, Aiping Lyu, William K Cheung*, Lu Zhang*

*Corresponding author for this work

Research output: Working paperPreprint

Abstract

Single-cell RNA sequencing is used to capture cell-specific gene expression, thus allowing reconstruction of gene regulatory networks. The existing algorithms struggle to deal with dropouts and cellular heterogeneity, and commonly require pseudotime-ordered cells. Here, we describe DeepDRIM a supervised deep neural network that represents gene pair joint expression as images and considers the neighborhood context to eliminate the transitive interactions. Deep-DRIM yields significantly better performance than the other nine algorithms used on the eight cell lines tested, and can be used to successfully discriminate key functional modules between patients with mild and severe symptoms of coronavirus disease 2019 (COVID-19).
Original languageEnglish
PublisherCold Spring Harbor Laboratory Press
Pages1-21
Number of pages21
DOIs
Publication statusPublished - 3 Feb 2021

Publication series

NamebioRxiv

User-Defined Keywords

  • single-cell RNA sequencing
  • gene regulatory network
  • deep neural network
  • transitive interactions

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