Decreased expression of J11d antigen during monocytic differentiation of M1 myeloid leukemic cells

M. C. Fung; Nai Ki Mak; K. N. Leung; A. J. Hapel

doi:10.1016/0008-8749(92)90132-9

Decreased expression of J11d antigen during monocytic differentiation of M1 myeloid leukemic cells

M. C. Fung^*, Nai Ki Mak, K. N. Leung, A. J. Hapel

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

10 Citations (Scopus)

Abstract

Four myeloid cell lines (M1, WEHI-3B D⁺, FDC-P1, and 32D) were screened for the presence of J11d antigen. One of these cell lines, the myeloid leukemia M1, was found to express a high level of J11d antigen on the cell surface. Recombinant mouse leukemic inhibitory factor (rm-LIF), recombinant human LIF (rh-LIF), and steroids (hydrocortisone and dexamethasone) could induce M1 cells to undergo monocytic differentiation. The level of J11d antigen was greatly reduced after treatment of the cells with LIF or steroids. Western blotting revealed that the apparent molecular weight of the J11d antigen on M1 cells was 45-48 kDa. Furthermore, the level of J11d mRNA was also reduced during LIF-induced differentiation of M1 cells.

Original language	English
Pages (from-to)	121-130
Number of pages	10
Journal	Cellular Immunology
Volume	141
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(92)90132-9
Publication status	Published - 15 Apr 1992

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Immunology

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title = "Decreased expression of J11d antigen during monocytic differentiation of M1 myeloid leukemic cells",

abstract = "Four myeloid cell lines (M1, WEHI-3B D+, FDC-P1, and 32D) were screened for the presence of J11d antigen. One of these cell lines, the myeloid leukemia M1, was found to express a high level of J11d antigen on the cell surface. Recombinant mouse leukemic inhibitory factor (rm-LIF), recombinant human LIF (rh-LIF), and steroids (hydrocortisone and dexamethasone) could induce M1 cells to undergo monocytic differentiation. The level of J11d antigen was greatly reduced after treatment of the cells with LIF or steroids. Western blotting revealed that the apparent molecular weight of the J11d antigen on M1 cells was 45-48 kDa. Furthermore, the level of J11d mRNA was also reduced during LIF-induced differentiation of M1 cells.",

author = "Fung, {M. C.} and Mak, {Nai Ki} and Leung, {K. N.} and Hapel, {A. J.}",

note = "Funding Information: {\textquoteright} To whom correspondence should be addressed. * Supported by grant (No. 900397) from NH & MRC of Australia. 3 Supported by a grant from the Commonwealth AIDS Research Grants Committee of Australia.",

year = "1992",

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day = "15",

doi = "10.1016/0008-8749(92)90132-9",

language = "English",

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T1 - Decreased expression of J11d antigen during monocytic differentiation of M1 myeloid leukemic cells

AU - Fung, M. C.

AU - Mak, Nai Ki

AU - Leung, K. N.

AU - Hapel, A. J.

N1 - Funding Information: ’ To whom correspondence should be addressed. * Supported by grant (No. 900397) from NH & MRC of Australia. 3 Supported by a grant from the Commonwealth AIDS Research Grants Committee of Australia.

PY - 1992/4/15

Y1 - 1992/4/15

N2 - Four myeloid cell lines (M1, WEHI-3B D+, FDC-P1, and 32D) were screened for the presence of J11d antigen. One of these cell lines, the myeloid leukemia M1, was found to express a high level of J11d antigen on the cell surface. Recombinant mouse leukemic inhibitory factor (rm-LIF), recombinant human LIF (rh-LIF), and steroids (hydrocortisone and dexamethasone) could induce M1 cells to undergo monocytic differentiation. The level of J11d antigen was greatly reduced after treatment of the cells with LIF or steroids. Western blotting revealed that the apparent molecular weight of the J11d antigen on M1 cells was 45-48 kDa. Furthermore, the level of J11d mRNA was also reduced during LIF-induced differentiation of M1 cells.

AB - Four myeloid cell lines (M1, WEHI-3B D+, FDC-P1, and 32D) were screened for the presence of J11d antigen. One of these cell lines, the myeloid leukemia M1, was found to express a high level of J11d antigen on the cell surface. Recombinant mouse leukemic inhibitory factor (rm-LIF), recombinant human LIF (rh-LIF), and steroids (hydrocortisone and dexamethasone) could induce M1 cells to undergo monocytic differentiation. The level of J11d antigen was greatly reduced after treatment of the cells with LIF or steroids. Western blotting revealed that the apparent molecular weight of the J11d antigen on M1 cells was 45-48 kDa. Furthermore, the level of J11d mRNA was also reduced during LIF-induced differentiation of M1 cells.

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U2 - 10.1016/0008-8749(92)90132-9

DO - 10.1016/0008-8749(92)90132-9

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AN - SCOPUS:0026520196

SN - 0008-8749

VL - 141

SP - 121

EP - 130

JO - Cellular Immunology

JF - Cellular Immunology

IS - 1

ER -

Decreased expression of J11d antigen during monocytic differentiation of M1 myeloid leukemic cells

Abstract

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