Decreased expression of J11d antigen during monocytic differentiation of M1 myeloid leukemic cells

M. C. Fung*, Nai Ki MAK, K. N. Leung, A. J. Hapel

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Four myeloid cell lines (M1, WEHI-3B D+, FDC-P1, and 32D) were screened for the presence of J11d antigen. One of these cell lines, the myeloid leukemia M1, was found to express a high level of J11d antigen on the cell surface. Recombinant mouse leukemic inhibitory factor (rm-LIF), recombinant human LIF (rh-LIF), and steroids (hydrocortisone and dexamethasone) could induce M1 cells to undergo monocytic differentiation. The level of J11d antigen was greatly reduced after treatment of the cells with LIF or steroids. Western blotting revealed that the apparent molecular weight of the J11d antigen on M1 cells was 45-48 kDa. Furthermore, the level of J11d mRNA was also reduced during LIF-induced differentiation of M1 cells.

Original languageEnglish
Pages (from-to)121-130
Number of pages10
JournalCellular Immunology
Volume141
Issue number1
DOIs
Publication statusPublished - 15 Apr 1992

Scopus Subject Areas

  • Immunology

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