TY - JOUR
T1 - Deciphering the molecular genetic basis of NPC through functional approaches
AU - Lung, Hong Lok
AU - Cheung, Arthur Kwok Leung
AU - Ko, Josephine Mun Yee
AU - Cheng, Yue
AU - Stanbridge, Eric J.
AU - Lung, Maria Li
N1 - Funding Information:
Hong Kong Research Grants Council General Research Grants HKU 661708M, HKU 661507M, HKUST 6611204M and Central Allocation Grant CA03/04.SC01 and University Grants Council Area of Excellence Grant AoE/M-06/08 to M.L.L.
Publisher copyright:
© 2011 Elsevier Ltd.
PY - 2012/4
Y1 - 2012/4
N2 - The identification of cancer genes in sporadic cancers has been recognized as a major challenge in the field. It is clear that deletion mapping, genomic sequencing, comparative genomic hybridization, or global gene expression profiling alone would not have easily identified candidate tumor suppressor genes (TSGs) from the huge array of lost regions or genes observed in nasopharyngeal carcinoma (NPC). In addition, the epigenetically silenced genes would not have been recognized by the mapping of deleted regions. In this review, we describe how functional approaches using monochromosome transfer may be used to circumvent the above problems and identify TSGs in NPC. A few examples of selected NPC TSGs and their functional roles are reviewed. They regulate a variety of gene functions including cell growth and proliferation, adhesion, migration, invasion, epithelial-mesenchymal transition, metastasis, and angiogenesis. These studies show the advantages of using functional approaches for identification of TSGs.
AB - The identification of cancer genes in sporadic cancers has been recognized as a major challenge in the field. It is clear that deletion mapping, genomic sequencing, comparative genomic hybridization, or global gene expression profiling alone would not have easily identified candidate tumor suppressor genes (TSGs) from the huge array of lost regions or genes observed in nasopharyngeal carcinoma (NPC). In addition, the epigenetically silenced genes would not have been recognized by the mapping of deleted regions. In this review, we describe how functional approaches using monochromosome transfer may be used to circumvent the above problems and identify TSGs in NPC. A few examples of selected NPC TSGs and their functional roles are reviewed. They regulate a variety of gene functions including cell growth and proliferation, adhesion, migration, invasion, epithelial-mesenchymal transition, metastasis, and angiogenesis. These studies show the advantages of using functional approaches for identification of TSGs.
KW - Microcell hybrid
KW - Microcell-mediated chromosome transfer
KW - Nasopharyngeal carcinoma
KW - Tumor segregant
KW - Tumor suppressor gene
UR - http://www.scopus.com/inward/record.url?scp=84857786329&partnerID=8YFLogxK
U2 - 10.1016/j.semcancer.2011.11.002
DO - 10.1016/j.semcancer.2011.11.002
M3 - Review article
C2 - 22154888
AN - SCOPUS:84857786329
SN - 1044-579X
VL - 22
SP - 87
EP - 95
JO - Seminars in Cancer Biology
JF - Seminars in Cancer Biology
IS - 2
ER -