De novo transcriptomic profile in the gonadal tissues of the intertidal whelk Reishia clavigera

Kevin K.Y. Ho; Priscilla T.Y. Leung; Jack C.H. Ip; J. W. Qiu; Kenneth M.Y. Leung

doi:10.1016/j.marpolbul.2014.02.023

De novo transcriptomic profile in the gonadal tissues of the intertidal whelk Reishia clavigera

Kevin K.Y. Ho, Priscilla T.Y. Leung, Jack C.H. Ip, J. W. Qiu, Kenneth M.Y. Leung^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

13 Citations (Scopus)

Abstract

The intertidal whelk Reishia clavigera (formerly named as Thais clavigera) is one of the most sensitive species to organotin-associated imposex. However, the limited information on mRNA transcriptome of the species has restricted the molecular investigation on such endocrine disruption. By means of Illumina sequencing, we obtained a global de novo transcriptome from the gonadal tissues of both male and female R. clavigera, with 197,324 assembled transcripts and 151,684 condensed non-redundant transcripts. Blast hit results from the NCBI's non-redundant molluscan database showed that 28,948 transcripts were successfully annotated with significant matches at an e-value of ≤1e^-6. Among them, 1108 transcripts were assigned a well-defined gene ontology term. As the first transcriptomic study on the gonadal tissues of R. clavigera, this study has enhanced the information of mRNA transcriptome on this species and will thus facilitate mechanistic studies of chemical contaminants (e.g., organotins) on this common biomonitor species.

Original language	English
Pages (from-to)	499-504
Number of pages	6
Journal	Marine Pollution Bulletin
Volume	85
Issue number	2
DOIs	https://doi.org/10.1016/j.marpolbul.2014.02.023
Publication status	Published - 30 Aug 2014

Scopus Subject Areas

Oceanography
Aquatic Science
Pollution

User-Defined Keywords

Endocrine disruption
Gastropod
Imposex
Organotin
Thais clavigera
Transcriptome

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.marpolbul.2014.02.023

Cite this

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title = "De novo transcriptomic profile in the gonadal tissues of the intertidal whelk Reishia clavigera",

abstract = "The intertidal whelk Reishia clavigera (formerly named as Thais clavigera) is one of the most sensitive species to organotin-associated imposex. However, the limited information on mRNA transcriptome of the species has restricted the molecular investigation on such endocrine disruption. By means of Illumina sequencing, we obtained a global de novo transcriptome from the gonadal tissues of both male and female R. clavigera, with 197,324 assembled transcripts and 151,684 condensed non-redundant transcripts. Blast hit results from the NCBI's non-redundant molluscan database showed that 28,948 transcripts were successfully annotated with significant matches at an e-value of ≤1e-6. Among them, 1108 transcripts were assigned a well-defined gene ontology term. As the first transcriptomic study on the gonadal tissues of R. clavigera, this study has enhanced the information of mRNA transcriptome on this species and will thus facilitate mechanistic studies of chemical contaminants (e.g., organotins) on this common biomonitor species.",

keywords = "Endocrine disruption, Gastropod, Imposex, Organotin, Thais clavigera, Transcriptome",

author = "Ho, {Kevin K.Y.} and Leung, {Priscilla T.Y.} and Ip, {Jack C.H.} and Qiu, {J. W.} and Leung, {Kenneth M.Y.}",

note = "Funding Information: This work was substantially supported by the Research Grants Council of Hong Kong SAR Government through a General Research Fund (Project no.: HKU 771212M). Priscilla Leung was partially supported by the State Key Laboratory in Marine Pollution via the Seed Collaborative Research Fund (2011) from Innovation and Technology Commission of the Hong Kong SAR Government. The authors thank the helpers who assisted in field sampling, Helen Leung and Cecily Law for their technical support, and Edward Lau for proofreading drafts of this manuscript. Kevin Ho and Jack Ip thank The University of Hong Kong for providing them postgraduate studentships. ",

year = "2014",

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AU - Ho, Kevin K.Y.

AU - Leung, Priscilla T.Y.

AU - Ip, Jack C.H.

AU - Qiu, J. W.

AU - Leung, Kenneth M.Y.

N1 - Funding Information: This work was substantially supported by the Research Grants Council of Hong Kong SAR Government through a General Research Fund (Project no.: HKU 771212M). Priscilla Leung was partially supported by the State Key Laboratory in Marine Pollution via the Seed Collaborative Research Fund (2011) from Innovation and Technology Commission of the Hong Kong SAR Government. The authors thank the helpers who assisted in field sampling, Helen Leung and Cecily Law for their technical support, and Edward Lau for proofreading drafts of this manuscript. Kevin Ho and Jack Ip thank The University of Hong Kong for providing them postgraduate studentships.

PY - 2014/8/30

Y1 - 2014/8/30

N2 - The intertidal whelk Reishia clavigera (formerly named as Thais clavigera) is one of the most sensitive species to organotin-associated imposex. However, the limited information on mRNA transcriptome of the species has restricted the molecular investigation on such endocrine disruption. By means of Illumina sequencing, we obtained a global de novo transcriptome from the gonadal tissues of both male and female R. clavigera, with 197,324 assembled transcripts and 151,684 condensed non-redundant transcripts. Blast hit results from the NCBI's non-redundant molluscan database showed that 28,948 transcripts were successfully annotated with significant matches at an e-value of ≤1e-6. Among them, 1108 transcripts were assigned a well-defined gene ontology term. As the first transcriptomic study on the gonadal tissues of R. clavigera, this study has enhanced the information of mRNA transcriptome on this species and will thus facilitate mechanistic studies of chemical contaminants (e.g., organotins) on this common biomonitor species.

AB - The intertidal whelk Reishia clavigera (formerly named as Thais clavigera) is one of the most sensitive species to organotin-associated imposex. However, the limited information on mRNA transcriptome of the species has restricted the molecular investigation on such endocrine disruption. By means of Illumina sequencing, we obtained a global de novo transcriptome from the gonadal tissues of both male and female R. clavigera, with 197,324 assembled transcripts and 151,684 condensed non-redundant transcripts. Blast hit results from the NCBI's non-redundant molluscan database showed that 28,948 transcripts were successfully annotated with significant matches at an e-value of ≤1e-6. Among them, 1108 transcripts were assigned a well-defined gene ontology term. As the first transcriptomic study on the gonadal tissues of R. clavigera, this study has enhanced the information of mRNA transcriptome on this species and will thus facilitate mechanistic studies of chemical contaminants (e.g., organotins) on this common biomonitor species.

KW - Endocrine disruption

KW - Gastropod

KW - Imposex

KW - Organotin

KW - Thais clavigera

KW - Transcriptome

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ER -

De novo transcriptomic profile in the gonadal tissues of the intertidal whelk Reishia clavigera

Abstract

Scopus Subject Areas

User-Defined Keywords

UN SDGs

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