De novo assembly of the transcriptome of an invasive snail and its multiple ecological applications

J. Sun, M. Wang, H. Wang, H. Zhang, X. Zhang, V. Thiyagarajan, P. Y. Qian, Jianwen QIU*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Studying how invasive species respond to environmental stress at the molecular level can help us assess their impact and predict their range expansion. Development of markers of genetic polymorphism can help us reconstruct their invasive route. However, to conduct such studies requires the presence of substantial amount of genomic resources. This study aimed to generate and characterize genomic resources using high throughput transcriptome sequencing for Pomacea canaliculata, a nonmodel gastropod indigenous to Argentina that has invaded Asia, Hawaii and southern United States. De novo assembly of the transcriptome resulted in 128 436 unigenes with an average length of 419 bp (range: 150-8556 bp). Many of the unigenes (2439) contained transposable elements, showing the existence of a source of genetic variability in response to stressful conditions. A total of 3196 microsatellites were detected in the transcriptome; among 20 of the randomly tested microsatellites, 10 were validated to exhibit polymorphism. A total of 15 412 single-nucleotide polymorphisms (SNPs) were detected in the ORFs. LC-MS/MS analysis of the proteome of juveniles revealed 878 proteins, of which many are stress related. This study has demonstrated the great potential of high throughput DNA sequencing for rapid development of genomic resources for a nonmodel organism. Such resources can facilitate various molecular ecological studies, such as stress physiology and range expansion.

Original languageEnglish
Pages (from-to)1133-1144
Number of pages12
JournalMolecular Ecology Resources
Volume12
Issue number6
DOIs
Publication statusPublished - Nov 2012

Scopus Subject Areas

  • Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics

User-Defined Keywords

  • Apple snail
  • Microsatellite
  • Pomacea
  • Proteome
  • Single-nucleotide polymorphism
  • Transcriptome

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