DANGER, a novel regulatory protein of inositol 1,4,5-trisphosphate-receptor activity

DB Van Rossum; RL Patterson; King-Ho CHEUNG; RK Barrow; V Syrovatkina; GS Gessell; SG Burkholder; DN Watkins; JK Foskett; SH Snyder

doi:10.1074/jbc.M608760200

DANGER, a novel regulatory protein of inositol 1,4,5-trisphosphate-receptor activity

DB Van Rossum, RL Patterson, King-Ho CHEUNG, RK Barrow, V Syrovatkina, GS Gessell, SG Burkholder, DN Watkins, JK Foskett, SH Snyder

Chinese Medicine - Teaching and Research Division

Research output: Contribution to journal › Journal article › peer-review

39 Citations (Scopus)

Abstract

We report the cloning and characterization of DANGER, a novel protein which physiologically binds to inositol 1,4,5-trisphosphate receptors (IP 3R). DANGER is a membrane-associated protein predicted to contain a partial MAB-21 domain. It is expressed in a wide variety of neuronal cell lineages where it localizes to membranes in the cell periphery together with IP3R. DANGER interacts with IP3R in vitro and co-immunoprecipitates with IP3R from cellular preparations. DANGER robustly enhances Ca2+-mediated inhibition of IP3R Ca 2+ release without affecting IP3 binding in microsomal assays and inhibits gating in single-channel recordings of IP3R. DANGER appears to allosterically modulate the sensitivity of IP3R to Ca2+ inhibition, which likely alters IP3R-mediated Ca 2+ dynamics in cells where DANGER and IP3R are co-expressed. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc.

Original language	English
Pages (from-to)	37111-37116
Number of pages	5
Journal	Journal of Biological Chemistry
Volume	281
Issue number	48
DOIs	https://doi.org/10.1074/jbc.M608760200
Publication status	Published - 2006

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title = "DANGER, a novel regulatory protein of inositol 1,4,5-trisphosphate-receptor activity",

abstract = "We report the cloning and characterization of DANGER, a novel protein which physiologically binds to inositol 1,4,5-trisphosphate receptors (IP 3R). DANGER is a membrane-associated protein predicted to contain a partial MAB-21 domain. It is expressed in a wide variety of neuronal cell lineages where it localizes to membranes in the cell periphery together with IP3R. DANGER interacts with IP3R in vitro and co-immunoprecipitates with IP3R from cellular preparations. DANGER robustly enhances Ca2+-mediated inhibition of IP3R Ca 2+ release without affecting IP3 binding in microsomal assays and inhibits gating in single-channel recordings of IP3R. DANGER appears to allosterically modulate the sensitivity of IP3R to Ca2+ inhibition, which likely alters IP3R-mediated Ca 2+ dynamics in cells where DANGER and IP3R are co-expressed. {\textcopyright} 2006 by The American Society for Biochemistry and Molecular Biology, Inc.",

author = "{Van Rossum}, DB and RL Patterson and King-Ho CHEUNG and RK Barrow and V Syrovatkina and GS Gessell and SG Burkholder and DN Watkins and JK Foskett and SH Snyder",

note = "This work was supported by United States Public Health Service Conte Center Grant MH 68830 (to S. H. S.), National Institutes of Health Grant GM/DK56328 (to J. K. F.), and Research Scientist Award DA-00074 (to S. H. S.) and by National Research Service Award NH65090 and the Searle Foundation (to R. L. P.). The costs of publication of this article were defrayed in part by the payment of page charges. ",

year = "2006",

doi = "10.1074/jbc.M608760200",

language = "English",

volume = "281",

pages = "37111--37116",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology Inc.",

number = "48",

}

TY - JOUR

T1 - DANGER, a novel regulatory protein of inositol 1,4,5-trisphosphate-receptor activity

AU - Van Rossum, DB

AU - Patterson, RL

AU - CHEUNG, King-Ho

AU - Barrow, RK

AU - Syrovatkina, V

AU - Gessell, GS

AU - Burkholder, SG

AU - Watkins, DN

AU - Foskett, JK

AU - Snyder, SH

N1 - This work was supported by United States Public Health Service Conte Center Grant MH 68830 (to S. H. S.), National Institutes of Health Grant GM/DK56328 (to J. K. F.), and Research Scientist Award DA-00074 (to S. H. S.) and by National Research Service Award NH65090 and the Searle Foundation (to R. L. P.). The costs of publication of this article were defrayed in part by the payment of page charges.

PY - 2006

Y1 - 2006

N2 - We report the cloning and characterization of DANGER, a novel protein which physiologically binds to inositol 1,4,5-trisphosphate receptors (IP 3R). DANGER is a membrane-associated protein predicted to contain a partial MAB-21 domain. It is expressed in a wide variety of neuronal cell lineages where it localizes to membranes in the cell periphery together with IP3R. DANGER interacts with IP3R in vitro and co-immunoprecipitates with IP3R from cellular preparations. DANGER robustly enhances Ca2+-mediated inhibition of IP3R Ca 2+ release without affecting IP3 binding in microsomal assays and inhibits gating in single-channel recordings of IP3R. DANGER appears to allosterically modulate the sensitivity of IP3R to Ca2+ inhibition, which likely alters IP3R-mediated Ca 2+ dynamics in cells where DANGER and IP3R are co-expressed. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc.

AB - We report the cloning and characterization of DANGER, a novel protein which physiologically binds to inositol 1,4,5-trisphosphate receptors (IP 3R). DANGER is a membrane-associated protein predicted to contain a partial MAB-21 domain. It is expressed in a wide variety of neuronal cell lineages where it localizes to membranes in the cell periphery together with IP3R. DANGER interacts with IP3R in vitro and co-immunoprecipitates with IP3R from cellular preparations. DANGER robustly enhances Ca2+-mediated inhibition of IP3R Ca 2+ release without affecting IP3 binding in microsomal assays and inhibits gating in single-channel recordings of IP3R. DANGER appears to allosterically modulate the sensitivity of IP3R to Ca2+ inhibition, which likely alters IP3R-mediated Ca 2+ dynamics in cells where DANGER and IP3R are co-expressed. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc.

U2 - 10.1074/jbc.M608760200

DO - 10.1074/jbc.M608760200

M3 - Journal article

SN - 0021-9258

VL - 281

SP - 37111

EP - 37116

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 48

ER -

DANGER, a novel regulatory protein of inositol 1,4,5-trisphosphate-receptor activity

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