TY - JOUR
T1 - Daily Exposure to a Cranberry Polyphenol Oral Rinse Alters the Oral Microbiome but Not Taste Perception in PROP Taster Status Classified Individuals
AU - Yousaf, Neeta Y.
AU - Wu, Guojun
AU - Melania, Melis
AU - Mariano, Mastinu
AU - Contini, Cristina
AU - Cabras, Tiziana
AU - Iole Tomassini, Barbarossa
AU - Zhao, Liping
AU - Lam, Yan Y
AU - Tepper, Beverly
N1 - Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Funding Information 1:
Funding: This work was supported by Rose Marie Pangborn Sensory Science Scholarship (2019 SSSF, awarded to N.Y.Y.), New Jersey Agricultural Experiment Station, HATCH Project 10180 and grants from the University of Cagliari: Fondi 5 per mille (Anno 2017, awarded to M.M. (Melania Melis)) and Fondo Integrativo per la Ricerca (FIR 2020 to I.T.B.). Additionally, we are grateful for the support of InnoHK (Hong Kong Special Administrative Region Government) to Y.Y.L.
Funding Information 2:
This work was supported by Rose Marie Pangborn Sensory Science Scholarship (2019 SSSF, awarded to N.Y.Y.), New Jersey Agricultural Experiment Station, HATCH Project 10180 and grants from the University of Cagliari: Fondi 5 per mille (Anno 2017, awarded to M.M. (Melania Melis)) and Fondo Integrativo per la Ricerca (FIR 2020 to I.T.B.). Additionally, we are grateful for the support of InnoHK (Hong Kong Special Administrative Region Government) to Y.Y.L.
PY - 2022/4/1
Y1 - 2022/4/1
N2 - Diet and salivary proteins influence the composition of the oral microbiome, and recent data suggest that TAS2R38 bitter taste genetics may also play a role. We investigated the effects of daily exposure to a cranberry polyphenol oral rinse on taste perception, salivary proteins, and oral microbiota. 6-n-Propylthiouracil (PROP) super-tasters (ST, n = 10) and non-tasters (NT, n = 10) rinsed with 30 mL of 0.75 g/L cranberry polyphenol extract (CPE) in spring water, twice daily for 11 days while consuming their habitual diets. The 16S rRNA gene sequencing showed that the NT oral microbiome composition was different than that of STs at baseline (p = 0.012) but not after the intervention (p = 0.525). Principal coordinates analysis using unweighted UniFrac distance showed that CPE modified microbiome composition in NTs (p = 0.023) but not in STs (p = 0.096). The intervention also altered specific salivary protein levels (α-amylase, MUC-5B, and selected S-type Cystatins) with no changes in sensory perception. Correlation networks between oral microbiota, salivary proteins, and sensory ratings showed that the ST microbiome had a more complex relationship with salivary proteins, particularly proline-rich proteins, than that in NTs. These findings show that CPE modulated the oral microbiome of NTs to be similar to that of STs, which could have implications for oral health.
AB - Diet and salivary proteins influence the composition of the oral microbiome, and recent data suggest that TAS2R38 bitter taste genetics may also play a role. We investigated the effects of daily exposure to a cranberry polyphenol oral rinse on taste perception, salivary proteins, and oral microbiota. 6-n-Propylthiouracil (PROP) super-tasters (ST, n = 10) and non-tasters (NT, n = 10) rinsed with 30 mL of 0.75 g/L cranberry polyphenol extract (CPE) in spring water, twice daily for 11 days while consuming their habitual diets. The 16S rRNA gene sequencing showed that the NT oral microbiome composition was different than that of STs at baseline (p = 0.012) but not after the intervention (p = 0.525). Principal coordinates analysis using unweighted UniFrac distance showed that CPE modified microbiome composition in NTs (p = 0.023) but not in STs (p = 0.096). The intervention also altered specific salivary protein levels (α-amylase, MUC-5B, and selected S-type Cystatins) with no changes in sensory perception. Correlation networks between oral microbiota, salivary proteins, and sensory ratings showed that the ST microbiome had a more complex relationship with salivary proteins, particularly proline-rich proteins, than that in NTs. These findings show that CPE modulated the oral microbiome of NTs to be similar to that of STs, which could have implications for oral health.
KW - 6-n-propylthiouracil
KW - cranberry polyphenols
KW - oral microbiota
KW - PROP phenotype
KW - salivary proteins
KW - TAS2R38
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85127394711&origin=inward
U2 - 10.3390/nu14071492
DO - 10.3390/nu14071492
M3 - Journal article
SN - 2072-6643
VL - 14
JO - Nutrients
JF - Nutrients
IS - 7
M1 - 1492
ER -