TY - JOUR
T1 - Cytosolic WPRa4 and Plastoskeletal PMI4 Proteins Mediate Touch Response in a Model Organism Arabidopsis
AU - Wu, Kebin
AU - Yang, Nan
AU - Ren, Jia
AU - Liu, Shichang
AU - Wang, Kai
AU - Dai, Shuaijian
AU - Lu, Yinglin
AU - An, Yuxing
AU - Tian, Fuyun
AU - Gao, Zhaobing
AU - Yang, Zhu
AU - Zhang, Yage
AU - Yu, Weichuan
AU - Li, Ning
N1 - https://www.mcponline.org/action/showPdf?pii=S1535947625001148
This work was supported by grants: 31370315, 31570187, 31870231, 32070205 from the National Science Foundation of China; 16102422, 16103621, 16101114, 16103817, 16103615, 16100318, 16101819, 16101920, 16306919, 12103820, R4012-18, C6021-19EF from the RGC of Hong Kong, ITS/480/18FP and MHP/033/20 from the Innovation and Technology Commission (ITC) of Hong Kong; Hetao Shenzhen-Hong Kong Science and Technology Innovation Cooperation Zone project (HZQB-KCZYB-2020083); High-level new R&D institute (No. 2019B090904008) and High-level Innovative Research Institute of Department of Science and Technology of Guangdong Province (No. 2021B0909050003); GDAS’Project of Science and Technology Development (2022GDASZH-2022010202); Foreign Expert Program from the Ministry of Science and Technology of China (G2022030053L), and the internal fund supports from HKUST; National Natural Science Foundation of China (Nos. 22302128), Guangdong Basic and Applied Basic Research Foundation (No. 2024A1515010990).
Publisher Copyright:
® 2025 THE AUTHORS.
Copyright © 2025 The Authors. Published by Elsevier Inc. All rights reserved.
PY - 2025/7
Y1 - 2025/7
N2 - Plastoskeletal PMI4 Protein is a Key Regulator of ThigmomorphogenesisTo elucidate the early signaling components involved in thigmomorphogenesis in Arabidopsis thaliana, we combined microscopy and proximity-labeling (PL)-based quantitative biotinylproteomics to characterize the touch-responsive putative cytoskeleton-interacting protein WPRa4 (TREPH1). Our findings revealed that WPRa4 localizes near plastids and interacts with cytosolic Plastid Movement-Impaired (PMI) proteins and a plastidic translocon component, suggesting a cytoskeleton-plastid network in mechanosensing. Bioinformatic analysis of PL and cross-linking mass spectrometry (XL-MS) data identified PMI4 as a key mediator, with pmi4 mutants lacking touch-induced bolting delay, rosette size reduction, and Ca
2+ oscillations. Transcriptomics further showed that PMI4 regulates touch-responsive and jasmonic acid (JA)-associated genes, such as LOX2. We propose a molecular model where interconnected Cytoskeleton-Plastoskeleton Continuum (CPC) proteins act as early mechanosensors, integrating the touch responses of plant aerial organs with calcium signaling and transcriptional reprogramming in Arabidopsis.
AB - Plastoskeletal PMI4 Protein is a Key Regulator of ThigmomorphogenesisTo elucidate the early signaling components involved in thigmomorphogenesis in Arabidopsis thaliana, we combined microscopy and proximity-labeling (PL)-based quantitative biotinylproteomics to characterize the touch-responsive putative cytoskeleton-interacting protein WPRa4 (TREPH1). Our findings revealed that WPRa4 localizes near plastids and interacts with cytosolic Plastid Movement-Impaired (PMI) proteins and a plastidic translocon component, suggesting a cytoskeleton-plastid network in mechanosensing. Bioinformatic analysis of PL and cross-linking mass spectrometry (XL-MS) data identified PMI4 as a key mediator, with pmi4 mutants lacking touch-induced bolting delay, rosette size reduction, and Ca
2+ oscillations. Transcriptomics further showed that PMI4 regulates touch-responsive and jasmonic acid (JA)-associated genes, such as LOX2. We propose a molecular model where interconnected Cytoskeleton-Plastoskeleton Continuum (CPC) proteins act as early mechanosensors, integrating the touch responses of plant aerial organs with calcium signaling and transcriptional reprogramming in Arabidopsis.
KW - Cytoskeleton-Plastoskeleton Continuum (CPC)
KW - Plastid Movement-Impaired 4 (PMI4/FtsZ1)
KW - Proximity Labeling (PL)
KW - Quantitative biotinylproteomics
KW - Retrograde calcium signal
KW - Thigmomorphogenesis
KW - Web1/PMI2-Related a4 (WPRa4/TREPH1)
UR - http://www.scopus.com/inward/record.url?scp=105012760444&partnerID=8YFLogxK
U2 - 10.1016/j.mcpro.2025.101015
DO - 10.1016/j.mcpro.2025.101015
M3 - Journal article
C2 - 40513778
SN - 1535-9476
VL - 24
JO - Molecular and Cellular Proteomics
JF - Molecular and Cellular Proteomics
IS - 7
M1 - 101015
ER -
