Cytosolic calcium during glucose deprivation in hippocampal pyramidal cells of rats

Thomas Knöpfel*, Andreas Spuler, Peter Grafe, Beat H. Gähwiler

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

30 Citations (Scopus)

Abstract

Glucose deprivation (GD) results in a hyperpolarization by turning on a potassium conductance (gK,GD) in hippocampal CA3 pyramidal cells. We used combined intracellular and microfluorometric recording techniques to evaluate whether gK,GD is activated by a rise in the concentration of intracellular calcium ([Ca2+]i). We found that the activation of gK,GD is only followed, but not preceded by a rise in [Ca2+]i. Furthermore, gK,GD is not blocked by the sulfonylurea glibenclamide, a blocker of ATP-regulated potassium conductance. We conclude that activation of gK,GD does not simply reflect breakdown of the calcium of ATP homeostasis, but on the contrary might represent an active restoring mechanism which delays the pathological consequences of sustained glucose deficiency.

Original languageEnglish
Pages (from-to)295-299
Number of pages5
JournalNeuroscience Letters
Volume117
Issue number3
DOIs
Publication statusPublished - 18 Sept 1990

User-Defined Keywords

  • Fura-2
  • Hippocampus
  • Hypoglycemia
  • Intracellular calcium
  • Intracellular recording
  • Sulfonylurea

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