Cyanines as new fluorescent probes for DNA detection and two-photon excited bioimaging

Xin Jiang Feng; Po Lam Wu; Frédéric Bolze; Heidi W.C. Leung; King Fai Li; Nai Ki MAK; Daniel W J KWONG; Jean François Nicoud; Kok Wai CHEAH; Ricky M S WONG

doi:10.1021/ol100474b

Cyanines as new fluorescent probes for DNA detection and two-photon excited bioimaging

Xin Jiang Feng, Po Lam Wu, Frédéric Bolze, Heidi W.C. Leung, King Fai Li, Nai Ki MAK, Daniel W J KWONG, Jean François Nicoud^*, Kok Wai CHEAH, Ricky M S WONG

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

113 Citations (Scopus)

Abstract

A series of cyanine fluorophores based on fused aromatics as an electron donor for DNA sensing and two-photon bioimaging were synthesized, among which the carbazole-based biscyanine exhibits high sensitivity and efficiency as a fluorescent light-up probe for dsDNA, which shows selective binding toward the AT-rich regions. The synergetic effect of the bischromophoric skeleton gives a several-fold enhancement in a two-photon absorption cross-section as well as a 25- to 100-fold enhancement in two-photon excited fluorescence upon dsDNA binding.

Original language	English
Pages (from-to)	2194-2197
Number of pages	4
Journal	Organic Letters
Volume	12
Issue number	10
DOIs	https://doi.org/10.1021/ol100474b
Publication status	Published - 21 May 2010

Scopus Subject Areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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10.1021/ol100474b

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@article{b11314f467a24a50a916c4538f79bd81,

title = "Cyanines as new fluorescent probes for DNA detection and two-photon excited bioimaging",

abstract = "A series of cyanine fluorophores based on fused aromatics as an electron donor for DNA sensing and two-photon bioimaging were synthesized, among which the carbazole-based biscyanine exhibits high sensitivity and efficiency as a fluorescent light-up probe for dsDNA, which shows selective binding toward the AT-rich regions. The synergetic effect of the bischromophoric skeleton gives a several-fold enhancement in a two-photon absorption cross-section as well as a 25- to 100-fold enhancement in two-photon excited fluorescence upon dsDNA binding.",

author = "Feng, {Xin Jiang} and Wu, {Po Lam} and Fr{\'e}d{\'e}ric Bolze and Leung, {Heidi W.C.} and Li, {King Fai} and MAK, {Nai Ki} and KWONG, {Daniel W J} and Nicoud, {Jean Fran{\c c}ois} and CHEAH, {Kok Wai} and WONG, {Ricky M S}",

note = "This work was financially supported by Hong Kong Research Grant Council, GRF, HKBU 202408, Hong Kong Baptist University, the Faculty Research Grant, FRG/08-09/II-16, and the PROCORE-France/Hong Kong Joint Research Scheme sponsored by the Research Grants Council of Hong Kong and the Consulate General of France in Hong Kong, F-HK04/07T. L. Richert and Y. M{\'e}ly at UdS are acknowledged for two-photon microscopy experiments. ",

year = "2010",

month = may,

day = "21",

doi = "10.1021/ol100474b",

language = "English",

volume = "12",

pages = "2194--2197",

journal = "Organic Letters",

issn = "1523-7060",

publisher = "American Chemical Society",

number = "10",

}

TY - JOUR

T1 - Cyanines as new fluorescent probes for DNA detection and two-photon excited bioimaging

AU - Feng, Xin Jiang

AU - Wu, Po Lam

AU - Bolze, Frédéric

AU - Leung, Heidi W.C.

AU - Li, King Fai

AU - MAK, Nai Ki

AU - KWONG, Daniel W J

AU - Nicoud, Jean François

AU - CHEAH, Kok Wai

AU - WONG, Ricky M S

N1 - This work was financially supported by Hong Kong Research Grant Council, GRF, HKBU 202408, Hong Kong Baptist University, the Faculty Research Grant, FRG/08-09/II-16, and the PROCORE-France/Hong Kong Joint Research Scheme sponsored by the Research Grants Council of Hong Kong and the Consulate General of France in Hong Kong, F-HK04/07T. L. Richert and Y. Mély at UdS are acknowledged for two-photon microscopy experiments.

PY - 2010/5/21

Y1 - 2010/5/21

N2 - A series of cyanine fluorophores based on fused aromatics as an electron donor for DNA sensing and two-photon bioimaging were synthesized, among which the carbazole-based biscyanine exhibits high sensitivity and efficiency as a fluorescent light-up probe for dsDNA, which shows selective binding toward the AT-rich regions. The synergetic effect of the bischromophoric skeleton gives a several-fold enhancement in a two-photon absorption cross-section as well as a 25- to 100-fold enhancement in two-photon excited fluorescence upon dsDNA binding.

AB - A series of cyanine fluorophores based on fused aromatics as an electron donor for DNA sensing and two-photon bioimaging were synthesized, among which the carbazole-based biscyanine exhibits high sensitivity and efficiency as a fluorescent light-up probe for dsDNA, which shows selective binding toward the AT-rich regions. The synergetic effect of the bischromophoric skeleton gives a several-fold enhancement in a two-photon absorption cross-section as well as a 25- to 100-fold enhancement in two-photon excited fluorescence upon dsDNA binding.

UR - http://www.scopus.com/inward/record.url?scp=77952325573&partnerID=8YFLogxK

U2 - 10.1021/ol100474b

DO - 10.1021/ol100474b

M3 - Journal article

C2 - 20405873

AN - SCOPUS:77952325573

SN - 1523-7060

VL - 12

SP - 2194

EP - 2197

JO - Organic Letters

JF - Organic Letters

IS - 10

ER -

Cyanines as new fluorescent probes for DNA detection and two-photon excited bioimaging

Abstract

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