Cyanine fluorophores for cellular protection against ROS in stimulated macrophages and two-photon ROS detection

M. S. Chan; D. Xu; L. Guo; D. Y. Tam; L. S. Liu; Y. Chen; M. S. Wong; P. K. Lo

doi:10.1039/c5ob00643k

Cyanine fluorophores for cellular protection against ROS in stimulated macrophages and two-photon ROS detection

M. S. Chan, D. Xu, L. Guo, D. Y. Tam, L. S. Liu, Y. Chen, M. S. Wong^*, P. K. Lo

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

15 Citations (Scopus)

Abstract

We report the first example of a novel two-photon active, biocompatible, and macrophage cell-membrane permeable carbazole-based cyanine fluorophore for the detection of three biologically important ROS, namely, ^•OH, O₂^- and OCl^- in solution. This versatile probe shows cellular protection not only in stimulated macrophages from phorbol-12-myristate-13-acetate-induced morphological changes but also lipopolysaccharide-induced cytotoxicity by quenching with the O₂^- and OCl^- production, respectively. Such protection could be visualized by a distinct change in the fluorescence intensity of the probe.

Original language	English
Pages (from-to)	7307-7312
Number of pages	6
Journal	Organic and Biomolecular Chemistry
Volume	13
Issue number	26
DOIs	https://doi.org/10.1039/c5ob00643k
Publication status	Published - 14 Jul 2015

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title = "Cyanine fluorophores for cellular protection against ROS in stimulated macrophages and two-photon ROS detection",

abstract = "We report the first example of a novel two-photon active, biocompatible, and macrophage cell-membrane permeable carbazole-based cyanine fluorophore for the detection of three biologically important ROS, namely, •OH, O2- and OCl- in solution. This versatile probe shows cellular protection not only in stimulated macrophages from phorbol-12-myristate-13-acetate-induced morphological changes but also lipopolysaccharide-induced cytotoxicity by quenching with the O2- and OCl- production, respectively. Such protection could be visualized by a distinct change in the fluorescence intensity of the probe.",

author = "Chan, {M. S.} and D. Xu and L. Guo and Tam, {D. Y.} and Liu, {L. S.} and Y. Chen and Wong, {M. S.} and Lo, {P. K.}",

note = "This work was financially supported by CityU Start-up Grant 7200300, CityU Strategic Research Grants 9616302 and 7004026, and National Science Foundation of China grant 21324077. This work was also supported by GRF (HKBU 203212), Hong Kong Research Grant Council and Institute of Molecular Functional Materials, which was supported by a grant from the University Grants Committee, Areas of Excellence Scheme (AoE/P-03/08).",

year = "2015",

month = jul,

day = "14",

doi = "10.1039/c5ob00643k",

language = "English",

volume = "13",

pages = "7307--7312",

journal = "Organic and Biomolecular Chemistry",

issn = "1477-0520",

publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - Cyanine fluorophores for cellular protection against ROS in stimulated macrophages and two-photon ROS detection

AU - Chan, M. S.

AU - Xu, D.

AU - Guo, L.

AU - Tam, D. Y.

AU - Liu, L. S.

AU - Chen, Y.

AU - Wong, M. S.

AU - Lo, P. K.

N1 - This work was financially supported by CityU Start-up Grant 7200300, CityU Strategic Research Grants 9616302 and 7004026, and National Science Foundation of China grant 21324077. This work was also supported by GRF (HKBU 203212), Hong Kong Research Grant Council and Institute of Molecular Functional Materials, which was supported by a grant from the University Grants Committee, Areas of Excellence Scheme (AoE/P-03/08).

PY - 2015/7/14

Y1 - 2015/7/14

N2 - We report the first example of a novel two-photon active, biocompatible, and macrophage cell-membrane permeable carbazole-based cyanine fluorophore for the detection of three biologically important ROS, namely, •OH, O2- and OCl- in solution. This versatile probe shows cellular protection not only in stimulated macrophages from phorbol-12-myristate-13-acetate-induced morphological changes but also lipopolysaccharide-induced cytotoxicity by quenching with the O2- and OCl- production, respectively. Such protection could be visualized by a distinct change in the fluorescence intensity of the probe.

AB - We report the first example of a novel two-photon active, biocompatible, and macrophage cell-membrane permeable carbazole-based cyanine fluorophore for the detection of three biologically important ROS, namely, •OH, O2- and OCl- in solution. This versatile probe shows cellular protection not only in stimulated macrophages from phorbol-12-myristate-13-acetate-induced morphological changes but also lipopolysaccharide-induced cytotoxicity by quenching with the O2- and OCl- production, respectively. Such protection could be visualized by a distinct change in the fluorescence intensity of the probe.

UR - http://www.scopus.com/inward/record.url?scp=84934916661&partnerID=8YFLogxK

U2 - 10.1039/c5ob00643k

DO - 10.1039/c5ob00643k

M3 - Journal article

C2 - 26059852

AN - SCOPUS:84934916661

SN - 1477-0520

VL - 13

SP - 7307

EP - 7312

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

IS - 26

ER -

Cyanine fluorophores for cellular protection against ROS in stimulated macrophages and two-photon ROS detection

Abstract

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