Cyanine fluorophores for cellular protection against ROS in stimulated macrophages and two-photon ROS detection

M. S. Chan; D. Xu; L. Guo; D. Y. Tam; L. S. Liu; Y. Chen; Ricky M S WONG; P. K. Lo

doi:10.1039/c5ob00643k

Cyanine fluorophores for cellular protection against ROS in stimulated macrophages and two-photon ROS detection

M. S. Chan, D. Xu, L. Guo, D. Y. Tam, L. S. Liu, Y. Chen, Ricky M S WONG^*, P. K. Lo

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

14 Citations (Scopus)

Abstract

We report the first example of a novel two-photon active, biocompatible, and macrophage cell-membrane permeable carbazole-based cyanine fluorophore for the detection of three biologically important ROS, namely, ^•OH, O₂^- and OCl^- in solution. This versatile probe shows cellular protection not only in stimulated macrophages from phorbol-12-myristate-13-acetate-induced morphological changes but also lipopolysaccharide-induced cytotoxicity by quenching with the O₂^- and OCl^- production, respectively. Such protection could be visualized by a distinct change in the fluorescence intensity of the probe.

Original language	English
Pages (from-to)	7307-7312
Number of pages	6
Journal	Organic and Biomolecular Chemistry
Volume	13
Issue number	26
DOIs	https://doi.org/10.1039/c5ob00643k
Publication status	Published - 14 Jul 2015

Scopus Subject Areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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title = "Cyanine fluorophores for cellular protection against ROS in stimulated macrophages and two-photon ROS detection",

abstract = "We report the first example of a novel two-photon active, biocompatible, and macrophage cell-membrane permeable carbazole-based cyanine fluorophore for the detection of three biologically important ROS, namely, •OH, O2- and OCl- in solution. This versatile probe shows cellular protection not only in stimulated macrophages from phorbol-12-myristate-13-acetate-induced morphological changes but also lipopolysaccharide-induced cytotoxicity by quenching with the O2- and OCl- production, respectively. Such protection could be visualized by a distinct change in the fluorescence intensity of the probe.",

author = "Chan, {M. S.} and D. Xu and L. Guo and Tam, {D. Y.} and Liu, {L. S.} and Y. Chen and WONG, {Ricky M S} and Lo, {P. K.}",

year = "2015",

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doi = "10.1039/c5ob00643k",

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TY - JOUR

T1 - Cyanine fluorophores for cellular protection against ROS in stimulated macrophages and two-photon ROS detection

AU - Chan, M. S.

AU - Xu, D.

AU - Guo, L.

AU - Tam, D. Y.

AU - Liu, L. S.

AU - Chen, Y.

AU - WONG, Ricky M S

AU - Lo, P. K.

PY - 2015/7/14

Y1 - 2015/7/14

N2 - We report the first example of a novel two-photon active, biocompatible, and macrophage cell-membrane permeable carbazole-based cyanine fluorophore for the detection of three biologically important ROS, namely, •OH, O2- and OCl- in solution. This versatile probe shows cellular protection not only in stimulated macrophages from phorbol-12-myristate-13-acetate-induced morphological changes but also lipopolysaccharide-induced cytotoxicity by quenching with the O2- and OCl- production, respectively. Such protection could be visualized by a distinct change in the fluorescence intensity of the probe.

AB - We report the first example of a novel two-photon active, biocompatible, and macrophage cell-membrane permeable carbazole-based cyanine fluorophore for the detection of three biologically important ROS, namely, •OH, O2- and OCl- in solution. This versatile probe shows cellular protection not only in stimulated macrophages from phorbol-12-myristate-13-acetate-induced morphological changes but also lipopolysaccharide-induced cytotoxicity by quenching with the O2- and OCl- production, respectively. Such protection could be visualized by a distinct change in the fluorescence intensity of the probe.

UR - http://www.scopus.com/inward/record.url?scp=84934916661&partnerID=8YFLogxK

U2 - 10.1039/c5ob00643k

DO - 10.1039/c5ob00643k

M3 - Journal article

C2 - 26059852

AN - SCOPUS:84934916661

SN - 1477-0520

VL - 13

SP - 7307

EP - 7312

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

IS - 26

ER -

Cyanine fluorophores for cellular protection against ROS in stimulated macrophages and two-photon ROS detection

Abstract

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