TY - JOUR
T1 - Cuticle thickness affects dynamics of volatile emission from petunia flowers
AU - Liao, Pan
AU - Ray, Shaunak
AU - Boachon, Benoît
AU - Lynch, Joseph H.
AU - Deshpande, Arnav
AU - McAdam, Scott
AU - Morgan, John A.
AU - Dudareva, Natalia
N1 - Funding Information:
This work was supported by grant from the National Science Foundation no. IOS-1655438 to N.D. and J.A.M. and by the USDA National Institute of Food and Agriculture Hatch Project no. 177845 to N.D. We acknowledge the use of the imaging facilities of the Bindley Bioscience Center, a core facility of the NIH-funded Indiana Clinical and Translational Sciences Institute, for collection of confocal microscopy images. We thank Y. Oshima (National Institute of Advanced Industrial Science and Technology, Japan) for providing pDONOR_P4PIR-InMYB1pro and R4pGWB5_stop_HSP vectors. We thank R. Seiler and L. Mueller for technical assistance on SEM and TEM, respectively.
Publisher Copyright:
© 2020, The Author(s), under exclusive licence to Springer Nature America, Inc.
PY - 2021/2
Y1 - 2021/2
N2 - The plant cuticle is the final barrier for volatile organic compounds
(VOCs) to cross for release to the atmosphere, yet its role in the
emission process is poorly understood. Here, using a combination of
reverse-genetic and chemical approaches, we demonstrate that the cuticle
imposes substantial resistance to VOC mass transfer, acting as a
sink/concentrator for VOCs and hence protecting cells from the
potentially toxic internal accumulation of these hydrophobic compounds.
Reduction in cuticle thickness has differential effects on individual
VOCs depending on their volatility, and leads to their internal cellular
redistribution, a shift in mass transfer resistance sources and altered
VOC synthesis. These results reveal that the cuticle is not simply a
passive diffusion barrier for VOCs to cross, but plays the
aforementioned complex roles in the emission process as an integral
member of the overall VOC network.
AB - The plant cuticle is the final barrier for volatile organic compounds
(VOCs) to cross for release to the atmosphere, yet its role in the
emission process is poorly understood. Here, using a combination of
reverse-genetic and chemical approaches, we demonstrate that the cuticle
imposes substantial resistance to VOC mass transfer, acting as a
sink/concentrator for VOCs and hence protecting cells from the
potentially toxic internal accumulation of these hydrophobic compounds.
Reduction in cuticle thickness has differential effects on individual
VOCs depending on their volatility, and leads to their internal cellular
redistribution, a shift in mass transfer resistance sources and altered
VOC synthesis. These results reveal that the cuticle is not simply a
passive diffusion barrier for VOCs to cross, but plays the
aforementioned complex roles in the emission process as an integral
member of the overall VOC network.
UR - http://www.scopus.com/inward/record.url?scp=85092708120&partnerID=8YFLogxK
U2 - 10.1038/s41589-020-00670-w
DO - 10.1038/s41589-020-00670-w
M3 - Journal article
C2 - 33077978
AN - SCOPUS:85092708120
SN - 1552-4450
VL - 17
SP - 138
EP - 145
JO - Nature Chemical Biology
JF - Nature Chemical Biology
IS - 2
ER -