Culture scale-up and immobilisation of a mixed methanotrophic consortium for methane remediation in pilot-scale bio-filters

Obulisamy Parthiba Karthikeyan; Nadarajan Saravanan; Samuel Cirés; Carlos Alvarez-Roa; Ali Razaghi; Karthigeyan Chidambarampadmavathy; Chinnathambi Velu; Gobalakrishnan Subashchandrabose; Kirsten Heimann

doi:10.1080/09593330.2016.1198424

Culture scale-up and immobilisation of a mixed methanotrophic consortium for methane remediation in pilot-scale bio-filters

Obulisamy Parthiba Karthikeyan
, Nadarajan Saravanan
, Samuel Cirés
, Carlos Alvarez-Roa
, Ali Razaghi
, Karthigeyan Chidambarampadmavathy
, Chinnathambi Velu
, Gobalakrishnan Subashchandrabose
, Kirsten Heimann^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

20 Citations (Scopus)

Abstract

Robust methanotrophic consortia for methane (CH₄) remediation and by-product development are presently not readily available for industrial use. In this study, a mixed methanotrophic consortium (MMC), sequentially enriched from a marine sediment, was assessed for CH₄removal efficiency and potential biomass-generated by-product development. Suitable packing material for bio-filters to support MMC biofilm establishment and growth was also evaluated. The enriched MMC removed ∼7–13% CH₄under a very high gas flow rate (2.5 L min⁻¹; 20–25% CH₄) in continuous-stirred tank reactors (∼10 L working volume) and the biomass contained long-chain fatty acids (i.e. C₁₆and C₁₈). Cultivation of the MMC on plastic bio-balls abated ∼95–97% CH₄in pilot-scale non-sterile outdoor-operated bio-filters (0.1 L min⁻¹; 1% CH₄). Contamination by cyanobacteria had beneficial effects on treating low-level CH₄, by providing additional oxygen for methane oxidation by MMC, suggesting that the co-cultivation of MMC with cyanobacterial mats does not interfere with and may actually be beneficial for remediation of CH₄and CO₂at industrial scale.

Original language	English
Pages (from-to)	474-482
Number of pages	9
Journal	Environmental Technology (United Kingdom)
Volume	38
Issue number	4
Early online date	5 Jul 2016
DOIs	https://doi.org/10.1080/09593330.2016.1198424
Publication status	Published - 16 Feb 2017

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

User-Defined Keywords

bio-ball
bio-filter
continuous-stirred tank reactor
marine sediment
Methane
methanotrophs

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10.1080/09593330.2016.1198424

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Karthikeyan, O. P., Saravanan, N., Cirés, S., Alvarez-Roa, C., Razaghi, A., Chidambarampadmavathy, K., Velu, C., Subashchandrabose, G., & Heimann, K. (2017). Culture scale-up and immobilisation of a mixed methanotrophic consortium for methane remediation in pilot-scale bio-filters. Environmental Technology (United Kingdom), 38(4), 474-482. https://doi.org/10.1080/09593330.2016.1198424

@article{d251cab901534288b6568a6a53c4953b,

title = "Culture scale-up and immobilisation of a mixed methanotrophic consortium for methane remediation in pilot-scale bio-filters",

abstract = "Robust methanotrophic consortia for methane (CH4) remediation and by-product development are presently not readily available for industrial use. In this study, a mixed methanotrophic consortium (MMC), sequentially enriched from a marine sediment, was assessed for CH4removal efficiency and potential biomass-generated by-product development. Suitable packing material for bio-filters to support MMC biofilm establishment and growth was also evaluated. The enriched MMC removed ∼7–13\% CH4under a very high gas flow rate (2.5 L min−1; 20–25\% CH4) in continuous-stirred tank reactors (∼10 L working volume) and the biomass contained long-chain fatty acids (i.e. C16and C18). Cultivation of the MMC on plastic bio-balls abated ∼95–97\% CH4in pilot-scale non-sterile outdoor-operated bio-filters (0.1 L min−1; 1\% CH4). Contamination by cyanobacteria had beneficial effects on treating low-level CH4, by providing additional oxygen for methane oxidation by MMC, suggesting that the co-cultivation of MMC with cyanobacterial mats does not interfere with and may actually be beneficial for remediation of CH4and CO2at industrial scale.",

keywords = "bio-ball, bio-filter, continuous-stirred tank reactor, marine sediment, Methane, methanotrophs",

author = "Karthikeyan, \{Obulisamy Parthiba\} and Nadarajan Saravanan and Samuel Cir{\'e}s and Carlos Alvarez-Roa and Ali Razaghi and Karthigeyan Chidambarampadmavathy and Chinnathambi Velu and Gobalakrishnan Subashchandrabose and Kirsten Heimann",

note = "The project was supported by the Advanced Manufacturing Cooperative Research Centre (AMCRC), funded through the Australian Government{\textquoteright}s Cooperative Research Centre Scheme [grant number 2.3.4]. Publisher Copyright: {\textcopyright} 2016 Informa UK Limited, trading as Taylor \& Francis Group.",

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doi = "10.1080/09593330.2016.1198424",

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Karthikeyan, OP, Saravanan, N, Cirés, S, Alvarez-Roa, C, Razaghi, A, Chidambarampadmavathy, K, Velu, C, Subashchandrabose, G & Heimann, K 2017, 'Culture scale-up and immobilisation of a mixed methanotrophic consortium for methane remediation in pilot-scale bio-filters', Environmental Technology (United Kingdom), vol. 38, no. 4, pp. 474-482. https://doi.org/10.1080/09593330.2016.1198424

TY - JOUR

T1 - Culture scale-up and immobilisation of a mixed methanotrophic consortium for methane remediation in pilot-scale bio-filters

AU - Karthikeyan, Obulisamy Parthiba

AU - Saravanan, Nadarajan

AU - Cirés, Samuel

AU - Alvarez-Roa, Carlos

AU - Razaghi, Ali

AU - Chidambarampadmavathy, Karthigeyan

AU - Velu, Chinnathambi

AU - Subashchandrabose, Gobalakrishnan

AU - Heimann, Kirsten

N1 - The project was supported by the Advanced Manufacturing Cooperative Research Centre (AMCRC), funded through the Australian Government’s Cooperative Research Centre Scheme [grant number 2.3.4]. Publisher Copyright: © 2016 Informa UK Limited, trading as Taylor & Francis Group.

PY - 2017/2/16

Y1 - 2017/2/16

N2 - Robust methanotrophic consortia for methane (CH4) remediation and by-product development are presently not readily available for industrial use. In this study, a mixed methanotrophic consortium (MMC), sequentially enriched from a marine sediment, was assessed for CH4removal efficiency and potential biomass-generated by-product development. Suitable packing material for bio-filters to support MMC biofilm establishment and growth was also evaluated. The enriched MMC removed ∼7–13% CH4under a very high gas flow rate (2.5 L min−1; 20–25% CH4) in continuous-stirred tank reactors (∼10 L working volume) and the biomass contained long-chain fatty acids (i.e. C16and C18). Cultivation of the MMC on plastic bio-balls abated ∼95–97% CH4in pilot-scale non-sterile outdoor-operated bio-filters (0.1 L min−1; 1% CH4). Contamination by cyanobacteria had beneficial effects on treating low-level CH4, by providing additional oxygen for methane oxidation by MMC, suggesting that the co-cultivation of MMC with cyanobacterial mats does not interfere with and may actually be beneficial for remediation of CH4and CO2at industrial scale.

AB - Robust methanotrophic consortia for methane (CH4) remediation and by-product development are presently not readily available for industrial use. In this study, a mixed methanotrophic consortium (MMC), sequentially enriched from a marine sediment, was assessed for CH4removal efficiency and potential biomass-generated by-product development. Suitable packing material for bio-filters to support MMC biofilm establishment and growth was also evaluated. The enriched MMC removed ∼7–13% CH4under a very high gas flow rate (2.5 L min−1; 20–25% CH4) in continuous-stirred tank reactors (∼10 L working volume) and the biomass contained long-chain fatty acids (i.e. C16and C18). Cultivation of the MMC on plastic bio-balls abated ∼95–97% CH4in pilot-scale non-sterile outdoor-operated bio-filters (0.1 L min−1; 1% CH4). Contamination by cyanobacteria had beneficial effects on treating low-level CH4, by providing additional oxygen for methane oxidation by MMC, suggesting that the co-cultivation of MMC with cyanobacterial mats does not interfere with and may actually be beneficial for remediation of CH4and CO2at industrial scale.

KW - bio-ball

KW - bio-filter

KW - continuous-stirred tank reactor

KW - marine sediment

KW - Methane

KW - methanotrophs

UR - https://www.scopus.com/pages/publications/84979010244

U2 - 10.1080/09593330.2016.1198424

DO - 10.1080/09593330.2016.1198424

M3 - Journal article

C2 - 27267085

AN - SCOPUS:84979010244

SN - 0959-3330

VL - 38

SP - 474

EP - 482

JO - Environmental Technology (United Kingdom)

JF - Environmental Technology (United Kingdom)

IS - 4

ER -

Culture scale-up and immobilisation of a mixed methanotrophic consortium for methane remediation in pilot-scale bio-filters

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