TY - JOUR
T1 - CTnI diagnosis in myocardial infarction using G-quadruplex selective Ir(Ⅲ) complex as effective electrochemiluminescence probe
AU - Wu, Shujie
AU - Zou, Seyin
AU - Wang, Songen
AU - Li, Zongbing
AU - Ma, Dik Lung
AU - Miao, Xiangmin
N1 - This work was supported by the Natural Science Foundation of Xuzhou City ( KC18140 ), the Hong Kong Baptist University ( FRG2/17–18/003 ), the Health and Medical Research Fund ( HMRF/14150561 ), the National Natural Science Foundation of China ( 21775131 , 21305053 ).
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/10/1
Y1 - 2022/10/1
N2 - In this work, strong electrochemiluminescence (ECL) emission was achieved by using one type of the G-quadruplex selective iridium (III) complex as an efficient ECL signal probe. Based on the typical sandwich immunoreaction between the cardiac troponin-I antigen (cTnI) and its corresponding antibody, iridium (III) complex was introduced according to its specific interaction with G-quadruplex DNA that modified on the surface of negatively charged gold nanoparticles ((−)AuNPs), inducing an increased ECL signal, which was proportional to cTnI concentration. Based on of this, quantitative detection of cTnI could be realized in the range of 5.0 fg/mL-100 ng/mL, with a detection limit of 1.67 fg/mL. Moreover, the proposed immunosensor was successfully applied for the diagnosis of cTnI in human serums from healthy individuals and acute myocardial infarction (AMI) patients, suggesting a great potential application value in the early diagnosis of AMI.
AB - In this work, strong electrochemiluminescence (ECL) emission was achieved by using one type of the G-quadruplex selective iridium (III) complex as an efficient ECL signal probe. Based on the typical sandwich immunoreaction between the cardiac troponin-I antigen (cTnI) and its corresponding antibody, iridium (III) complex was introduced according to its specific interaction with G-quadruplex DNA that modified on the surface of negatively charged gold nanoparticles ((−)AuNPs), inducing an increased ECL signal, which was proportional to cTnI concentration. Based on of this, quantitative detection of cTnI could be realized in the range of 5.0 fg/mL-100 ng/mL, with a detection limit of 1.67 fg/mL. Moreover, the proposed immunosensor was successfully applied for the diagnosis of cTnI in human serums from healthy individuals and acute myocardial infarction (AMI) patients, suggesting a great potential application value in the early diagnosis of AMI.
KW - cTnI detection
KW - Electrochemiluminescence
KW - G-quadruplex
KW - Gold nanoparticles
KW - Iridium(III) complex
UR - http://www.scopus.com/inward/record.url?scp=85131432594&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2022.123622
DO - 10.1016/j.talanta.2022.123622
M3 - Journal article
C2 - 35687951
AN - SCOPUS:85131432594
SN - 0039-9140
VL - 248
JO - Talanta
JF - Talanta
M1 - 123622
ER -
