CTnI diagnosis in myocardial infarction using G-quadruplex selective Ir(Ⅲ) complex as effective electrochemiluminescence probe

Shujie Wu; Seyin Zou; Songen Wang; Zongbing Li; Dik Lung Ma; Xiangmin Miao

doi:10.1016/j.talanta.2022.123622

CTnI diagnosis in myocardial infarction using G-quadruplex selective Ir(Ⅲ) complex as effective electrochemiluminescence probe

Shujie Wu, Seyin Zou, Songen Wang, Zongbing Li, Dik Lung Ma^*, Xiangmin Miao^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

14 Citations (Scopus)

Abstract

In this work, strong electrochemiluminescence (ECL) emission was achieved by using one type of the G-quadruplex selective iridium (III) complex as an efficient ECL signal probe. Based on the typical sandwich immunoreaction between the cardiac troponin-I antigen (cTnI) and its corresponding antibody, iridium (III) complex was introduced according to its specific interaction with G-quadruplex DNA that modified on the surface of negatively charged gold nanoparticles ((−)AuNPs), inducing an increased ECL signal, which was proportional to cTnI concentration. Based on of this, quantitative detection of cTnI could be realized in the range of 5.0 fg/mL-100 ng/mL, with a detection limit of 1.67 fg/mL. Moreover, the proposed immunosensor was successfully applied for the diagnosis of cTnI in human serums from healthy individuals and acute myocardial infarction (AMI) patients, suggesting a great potential application value in the early diagnosis of AMI.

Original language	English
Article number	123622
Journal	Talanta
Volume	248
DOIs	https://doi.org/10.1016/j.talanta.2022.123622
Publication status	Published - 1 Oct 2022

User-Defined Keywords

cTnI detection
Electrochemiluminescence
G-quadruplex
Gold nanoparticles
Iridium(III) complex

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10.1016/j.talanta.2022.123622

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title = "CTnI diagnosis in myocardial infarction using G-quadruplex selective Ir(Ⅲ) complex as effective electrochemiluminescence probe",

abstract = "In this work, strong electrochemiluminescence (ECL) emission was achieved by using one type of the G-quadruplex selective iridium (III) complex as an efficient ECL signal probe. Based on the typical sandwich immunoreaction between the cardiac troponin-I antigen (cTnI) and its corresponding antibody, iridium (III) complex was introduced according to its specific interaction with G-quadruplex DNA that modified on the surface of negatively charged gold nanoparticles ((−)AuNPs), inducing an increased ECL signal, which was proportional to cTnI concentration. Based on of this, quantitative detection of cTnI could be realized in the range of 5.0 fg/mL-100 ng/mL, with a detection limit of 1.67 fg/mL. Moreover, the proposed immunosensor was successfully applied for the diagnosis of cTnI in human serums from healthy individuals and acute myocardial infarction (AMI) patients, suggesting a great potential application value in the early diagnosis of AMI.",

keywords = "cTnI detection, Electrochemiluminescence, G-quadruplex, Gold nanoparticles, Iridium(III) complex",

author = "Shujie Wu and Seyin Zou and Songen Wang and Zongbing Li and Ma, {Dik Lung} and Xiangmin Miao",

note = "This work was supported by the Natural Science Foundation of Xuzhou City ( KC18140 ), the Hong Kong Baptist University ( FRG2/17–18/003 ), the Health and Medical Research Fund ( HMRF/14150561 ), the National Natural Science Foundation of China ( 21775131 , 21305053 ). Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",

year = "2022",

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language = "English",

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T1 - CTnI diagnosis in myocardial infarction using G-quadruplex selective Ir(Ⅲ) complex as effective electrochemiluminescence probe

AU - Wu, Shujie

AU - Zou, Seyin

AU - Wang, Songen

AU - Li, Zongbing

AU - Ma, Dik Lung

AU - Miao, Xiangmin

N1 - This work was supported by the Natural Science Foundation of Xuzhou City ( KC18140 ), the Hong Kong Baptist University ( FRG2/17–18/003 ), the Health and Medical Research Fund ( HMRF/14150561 ), the National Natural Science Foundation of China ( 21775131 , 21305053 ). Publisher Copyright: © 2022 Elsevier B.V.

PY - 2022/10/1

Y1 - 2022/10/1

N2 - In this work, strong electrochemiluminescence (ECL) emission was achieved by using one type of the G-quadruplex selective iridium (III) complex as an efficient ECL signal probe. Based on the typical sandwich immunoreaction between the cardiac troponin-I antigen (cTnI) and its corresponding antibody, iridium (III) complex was introduced according to its specific interaction with G-quadruplex DNA that modified on the surface of negatively charged gold nanoparticles ((−)AuNPs), inducing an increased ECL signal, which was proportional to cTnI concentration. Based on of this, quantitative detection of cTnI could be realized in the range of 5.0 fg/mL-100 ng/mL, with a detection limit of 1.67 fg/mL. Moreover, the proposed immunosensor was successfully applied for the diagnosis of cTnI in human serums from healthy individuals and acute myocardial infarction (AMI) patients, suggesting a great potential application value in the early diagnosis of AMI.

AB - In this work, strong electrochemiluminescence (ECL) emission was achieved by using one type of the G-quadruplex selective iridium (III) complex as an efficient ECL signal probe. Based on the typical sandwich immunoreaction between the cardiac troponin-I antigen (cTnI) and its corresponding antibody, iridium (III) complex was introduced according to its specific interaction with G-quadruplex DNA that modified on the surface of negatively charged gold nanoparticles ((−)AuNPs), inducing an increased ECL signal, which was proportional to cTnI concentration. Based on of this, quantitative detection of cTnI could be realized in the range of 5.0 fg/mL-100 ng/mL, with a detection limit of 1.67 fg/mL. Moreover, the proposed immunosensor was successfully applied for the diagnosis of cTnI in human serums from healthy individuals and acute myocardial infarction (AMI) patients, suggesting a great potential application value in the early diagnosis of AMI.

KW - cTnI detection

KW - Electrochemiluminescence

KW - G-quadruplex

KW - Gold nanoparticles

KW - Iridium(III) complex

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U2 - 10.1016/j.talanta.2022.123622

DO - 10.1016/j.talanta.2022.123622

M3 - Journal article

C2 - 35687951

AN - SCOPUS:85131432594

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VL - 248

JO - Talanta

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ER -

CTnI diagnosis in myocardial infarction using G-quadruplex selective Ir(Ⅲ) complex as effective electrochemiluminescence probe

Abstract

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