Coupling a microchip with electrospray ionization quadrupole time-of-flight mass spectrometer for peptide separation and identification

Hai Fang Li, Jiangjiang Liu, Zongwei CAI, Jin Ming Lin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The present study reports a simple method of coupling a glass microchip to an electrospray ionization (ESI) quadrupole time-of-flight mass spectrometer (QTOF-MS) for separation and identification of peptides. A sheath-flow electrospray interface was constructed based on attaching a short fused-silica capillary to the microchip. The dead volume at the interface was effectively reduced by wet etching an approximate flat-bottom capillary insertion channel coaxial to the end of separation microchannel and using a wire-controlled epoxy-blocking attachment method. The makeup liquid and neb gas were coaxially pumped through two stainless-steel tees to maintain a stable and efficient electrospray. The coupled microchip/ESI-QTOF-MS system was successfully used to carry out electrophoresis separation of peptides and ESI-QTOF-MS identification.

Original languageEnglish
Pages (from-to)1889-1894
Number of pages6
JournalElectrophoresis
Volume29
Issue number9
DOIs
Publication statusPublished - May 2008

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

User-Defined Keywords

  • Electrospray ionization
  • Microchip electrophoresis
  • Peptides
  • Quadrupole time-of-flight mass spectrometer

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