Abstract
The present study reports a simple method of coupling a glass microchip to an electrospray ionization (ESI) quadrupole time-of-flight mass spectrometer (QTOF-MS) for separation and identification of peptides. A sheath-flow electrospray interface was constructed based on attaching a short fused-silica capillary to the microchip. The dead volume at the interface was effectively reduced by wet etching an approximate flat-bottom capillary insertion channel coaxial to the end of separation microchannel and using a wire-controlled epoxy-blocking attachment method. The makeup liquid and neb gas were coaxially pumped through two stainless-steel tees to maintain a stable and efficient electrospray. The coupled microchip/ESI-QTOF-MS system was successfully used to carry out electrophoresis separation of peptides and ESI-QTOF-MS identification.
Original language | English |
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Pages (from-to) | 1889-1894 |
Number of pages | 6 |
Journal | Electrophoresis |
Volume | 29 |
Issue number | 9 |
DOIs | |
Publication status | Published - May 2008 |
User-Defined Keywords
- Electrospray ionization
- Microchip electrophoresis
- Peptides
- Quadrupole time-of-flight mass spectrometer