Cordyceps polysaccharide marker CCP modulates immune responses via highly selective TLR4/MyD88/p38 axis

Quanwei Zhang; Man Liu; Lifeng Li; Miaomiao Chen; Pema Tenzin Puno; Wanrong Bao; Hongming Zheng; Xin Wen; Huiyuan Cheng; Hauyee Fung; Tinlong Wong; Zhongzhen Zhao; Aiping Lyu; Quanbin Han; Handong Sun

doi:10.1016/j.carbpol.2021.118443

Cordyceps polysaccharide marker CCP modulates immune responses via highly selective TLR4/MyD88/p38 axis

Quanwei Zhang, Man Liu, Lifeng Li, Miaomiao Chen, Pema Tenzin Puno, Wanrong Bao, Hongming Zheng, Xin Wen, Huiyuan Cheng, Hauyee Fung, Tinlong Wong, Zhongzhen Zhao, Aiping Lyu^*, Quanbin Han^*, Handong Sun

^*Corresponding author for this work

School of Chinese Medicine

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Cordyceps, one of the most expensive natural health supplements, is popularly used to modulate immune function. However, little is known regarding the underlying mechanism of its immunomodulatory activity. We newly reported a Cordyceps quality marker CCP (Mw 433.778 kDa) which was characterized as a 1,4-α glucan by chemical and spectral analysis and is able to induce significant immune responses of macrophages. Herein, we further investigated the molecular mechanism of CCP's immunomodulatory effects. The results indicate that CCP modulates the TLR4/MyD88/p38 signaling pathway of macrophages, where TLR4 plays a crucial role as verified on TLR4-deficient (TLR4^−/−) bone marrow-derived macrophages (BMDMs) and TLR4^−/− mice. These findings provide a precise understanding of the molecular mechanism of Cordyceps' immunomodulatory benefits.

Original language	English
Article number	118443
Number of pages	11
Journal	Carbohydrate Polymers
Volume	271
Early online date	16 Jul 2021
DOIs	https://doi.org/10.1016/j.carbpol.2021.118443
Publication status	Published - 1 Nov 2021

Scopus Subject Areas

Organic Chemistry
Polymers and Plastics
Materials Chemistry

User-Defined Keywords

Cordyceps
Immune responses
Macrophage
Polysaccharide
TLR4/MyD88/p38

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10.1016/j.carbpol.2021.118443

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@article{ddab2fa9f1c4447888ab2fc5f57c2819,

title = "Cordyceps polysaccharide marker CCP modulates immune responses via highly selective TLR4/MyD88/p38 axis",

abstract = "Cordyceps, one of the most expensive natural health supplements, is popularly used to modulate immune function. However, little is known regarding the underlying mechanism of its immunomodulatory activity. We newly reported a Cordyceps quality marker CCP (Mw 433.778 kDa) which was characterized as a 1,4-α glucan by chemical and spectral analysis and is able to induce significant immune responses of macrophages. Herein, we further investigated the molecular mechanism of CCP's immunomodulatory effects. The results indicate that CCP modulates the TLR4/MyD88/p38 signaling pathway of macrophages, where TLR4 plays a crucial role as verified on TLR4-deficient (TLR4−/−) bone marrow-derived macrophages (BMDMs) and TLR4−/− mice. These findings provide a precise understanding of the molecular mechanism of Cordyceps' immunomodulatory benefits.",

keywords = "Cordyceps, Immune responses, Macrophage, Polysaccharide, TLR4/MyD88/p38",

author = "Quanwei Zhang and Man Liu and Lifeng Li and Miaomiao Chen and Puno, {Pema Tenzin} and Wanrong Bao and Hongming Zheng and Xin Wen and Huiyuan Cheng and Hauyee Fung and Tinlong Wong and Zhongzhen Zhao and Aiping Lyu and Quanbin Han and Handong Sun",

note = "Funding Information: This work was funded and supported by HKSAR Innovation and Technology Fund (ITF), Tier 3, ITS/311/09 , General Research Fund ( 12100615 , 22100014 , 12100818 ), UGC Research Matching Grant Scheme ( 2019-1-10 , 2019-1-14 , 2019-2-06 ), Health Medical Research Fund ( 11122531 , 14150521 , 17182681 ), National Natural Sciences Foundation in China ( 81473341 ), the Science and Technology Project of Shenzhen ( JCYJ20160531193812867 ), the Key-Area Research and Development Program of Guangdong Province ( 2020B1111110007 ), Hong Kong Baptist University (RC-Start-up Grants, MPCF-001-2016/2017 , RC-IRMS/14-15/06 , FRG2/17-18/060 and FRG2/16-17/002 ), and Vincent & Lily Woo Foundation . Publisher Copyright: {\textcopyright} 2021",

year = "2021",

month = nov,

day = "1",

doi = "10.1016/j.carbpol.2021.118443",

language = "English",

volume = "271",

journal = "Carbohydrate Polymers",

issn = "0144-8617",

publisher = "Elsevier Ltd.",

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TY - JOUR

T1 - Cordyceps polysaccharide marker CCP modulates immune responses via highly selective TLR4/MyD88/p38 axis

AU - Zhang, Quanwei

AU - Liu, Man

AU - Li, Lifeng

AU - Chen, Miaomiao

AU - Puno, Pema Tenzin

AU - Bao, Wanrong

AU - Zheng, Hongming

AU - Wen, Xin

AU - Cheng, Huiyuan

AU - Fung, Hauyee

AU - Wong, Tinlong

AU - Zhao, Zhongzhen

AU - Lyu, Aiping

AU - Han, Quanbin

AU - Sun, Handong

N1 - Funding Information: This work was funded and supported by HKSAR Innovation and Technology Fund (ITF), Tier 3, ITS/311/09 , General Research Fund ( 12100615 , 22100014 , 12100818 ), UGC Research Matching Grant Scheme ( 2019-1-10 , 2019-1-14 , 2019-2-06 ), Health Medical Research Fund ( 11122531 , 14150521 , 17182681 ), National Natural Sciences Foundation in China ( 81473341 ), the Science and Technology Project of Shenzhen ( JCYJ20160531193812867 ), the Key-Area Research and Development Program of Guangdong Province ( 2020B1111110007 ), Hong Kong Baptist University (RC-Start-up Grants, MPCF-001-2016/2017 , RC-IRMS/14-15/06 , FRG2/17-18/060 and FRG2/16-17/002 ), and Vincent & Lily Woo Foundation . Publisher Copyright: © 2021

PY - 2021/11/1

Y1 - 2021/11/1

N2 - Cordyceps, one of the most expensive natural health supplements, is popularly used to modulate immune function. However, little is known regarding the underlying mechanism of its immunomodulatory activity. We newly reported a Cordyceps quality marker CCP (Mw 433.778 kDa) which was characterized as a 1,4-α glucan by chemical and spectral analysis and is able to induce significant immune responses of macrophages. Herein, we further investigated the molecular mechanism of CCP's immunomodulatory effects. The results indicate that CCP modulates the TLR4/MyD88/p38 signaling pathway of macrophages, where TLR4 plays a crucial role as verified on TLR4-deficient (TLR4−/−) bone marrow-derived macrophages (BMDMs) and TLR4−/− mice. These findings provide a precise understanding of the molecular mechanism of Cordyceps' immunomodulatory benefits.

AB - Cordyceps, one of the most expensive natural health supplements, is popularly used to modulate immune function. However, little is known regarding the underlying mechanism of its immunomodulatory activity. We newly reported a Cordyceps quality marker CCP (Mw 433.778 kDa) which was characterized as a 1,4-α glucan by chemical and spectral analysis and is able to induce significant immune responses of macrophages. Herein, we further investigated the molecular mechanism of CCP's immunomodulatory effects. The results indicate that CCP modulates the TLR4/MyD88/p38 signaling pathway of macrophages, where TLR4 plays a crucial role as verified on TLR4-deficient (TLR4−/−) bone marrow-derived macrophages (BMDMs) and TLR4−/− mice. These findings provide a precise understanding of the molecular mechanism of Cordyceps' immunomodulatory benefits.

KW - Cordyceps

KW - Immune responses

KW - Macrophage

KW - Polysaccharide

KW - TLR4/MyD88/p38

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U2 - 10.1016/j.carbpol.2021.118443

DO - 10.1016/j.carbpol.2021.118443

M3 - Article

C2 - 34364580

AN - SCOPUS:85111013816

SN - 0144-8617

VL - 271

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

M1 - 118443

ER -

Cordyceps polysaccharide marker CCP modulates immune responses via highly selective TLR4/MyD88/p38 axis

Abstract

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