Comprehensive identification of active triterpenoid metabolites in frankincense using a coupling strategy

Yuanyan Liu; Zhenli Liu; Cheng Lu; Jianbei Li; Zhangchi Ning; Zhiqian Song; Chun Wang; Zhiyong Du; Xinmiao Lu; Siyu Zhao; Aiping LYU

doi:10.1016/j.jchromb.2014.05.054

Comprehensive identification of active triterpenoid metabolites in frankincense using a coupling strategy

Yuanyan Liu^*, Zhenli Liu, Cheng Lu, Jianbei Li, Zhangchi Ning, Zhiqian Song, Chun Wang, Zhiyong Du, Xinmiao Lu, Siyu Zhao, Aiping LYU

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

8 Citations (Scopus)

Abstract

Frankincense resins are extensively used as natural remedies in regions ranging from North Africa to China. Triterpenoid metabolites from frankincense exhibit notable anti-inflammatory and anti-tumor properties. In the present paper, without the use of an isolation process, the fragmentation rules and NMR spectral characteristics of triterpenoid metabolites in frankincense are summarized through a coupling method using high performance liquid chromatography-diode array detection/electrospray ionization tandem mass spectrometry (HPLC-DAD/ESI-MSⁿ) combined with HPLC-nuclear magnetic resonance (NMR) experiments. Based on this groundwork, a coupling strategy for the comprehensive metabolic profiling of active triterpenoid metabolites from enriched fractions of frankincense was developed. The proposed strategy may serve as a method for the holistic screening of bioactive metabolites in complex TCM samples.

Original language	English
Pages (from-to)	90-98
Number of pages	9
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	963
DOIs	https://doi.org/10.1016/j.jchromb.2014.05.054
Publication status	Published - 15 Jul 2014

Scopus Subject Areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Cell Biology

User-Defined Keywords

Frankincense resins
Mass spectrometry
Metabolic profiling
Nuclear magnetic resonance
Triterpenoid metabolites

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10.1016/j.jchromb.2014.05.054

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title = "Comprehensive identification of active triterpenoid metabolites in frankincense using a coupling strategy",

abstract = "Frankincense resins are extensively used as natural remedies in regions ranging from North Africa to China. Triterpenoid metabolites from frankincense exhibit notable anti-inflammatory and anti-tumor properties. In the present paper, without the use of an isolation process, the fragmentation rules and NMR spectral characteristics of triterpenoid metabolites in frankincense are summarized through a coupling method using high performance liquid chromatography-diode array detection/electrospray ionization tandem mass spectrometry (HPLC-DAD/ESI-MSn) combined with HPLC-nuclear magnetic resonance (NMR) experiments. Based on this groundwork, a coupling strategy for the comprehensive metabolic profiling of active triterpenoid metabolites from enriched fractions of frankincense was developed. The proposed strategy may serve as a method for the holistic screening of bioactive metabolites in complex TCM samples.",

keywords = "Frankincense resins, Mass spectrometry, Metabolic profiling, Nuclear magnetic resonance, Triterpenoid metabolites",

author = "Yuanyan Liu and Zhenli Liu and Cheng Lu and Jianbei Li and Zhangchi Ning and Zhiqian Song and Chun Wang and Zhiyong Du and Xinmiao Lu and Siyu Zhao and Aiping LYU",

note = "Funding Information: This study was financially supported by the National Science Foundation of China (Project No. 30902000 and No. 81001623 ), the Fundamental Research Funds for the Central public welfare research institutes (Project No. YZ-1412) and the National Basic Research Program of China (Project No. 2012CB518401). ",

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doi = "10.1016/j.jchromb.2014.05.054",

language = "English",

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AU - Liu, Yuanyan

AU - Liu, Zhenli

AU - Lu, Cheng

AU - Li, Jianbei

AU - Ning, Zhangchi

AU - Song, Zhiqian

AU - Wang, Chun

AU - Du, Zhiyong

AU - Lu, Xinmiao

AU - Zhao, Siyu

AU - LYU, Aiping

N1 - Funding Information: This study was financially supported by the National Science Foundation of China (Project No. 30902000 and No. 81001623 ), the Fundamental Research Funds for the Central public welfare research institutes (Project No. YZ-1412) and the National Basic Research Program of China (Project No. 2012CB518401).

PY - 2014/7/15

Y1 - 2014/7/15

N2 - Frankincense resins are extensively used as natural remedies in regions ranging from North Africa to China. Triterpenoid metabolites from frankincense exhibit notable anti-inflammatory and anti-tumor properties. In the present paper, without the use of an isolation process, the fragmentation rules and NMR spectral characteristics of triterpenoid metabolites in frankincense are summarized through a coupling method using high performance liquid chromatography-diode array detection/electrospray ionization tandem mass spectrometry (HPLC-DAD/ESI-MSn) combined with HPLC-nuclear magnetic resonance (NMR) experiments. Based on this groundwork, a coupling strategy for the comprehensive metabolic profiling of active triterpenoid metabolites from enriched fractions of frankincense was developed. The proposed strategy may serve as a method for the holistic screening of bioactive metabolites in complex TCM samples.

AB - Frankincense resins are extensively used as natural remedies in regions ranging from North Africa to China. Triterpenoid metabolites from frankincense exhibit notable anti-inflammatory and anti-tumor properties. In the present paper, without the use of an isolation process, the fragmentation rules and NMR spectral characteristics of triterpenoid metabolites in frankincense are summarized through a coupling method using high performance liquid chromatography-diode array detection/electrospray ionization tandem mass spectrometry (HPLC-DAD/ESI-MSn) combined with HPLC-nuclear magnetic resonance (NMR) experiments. Based on this groundwork, a coupling strategy for the comprehensive metabolic profiling of active triterpenoid metabolites from enriched fractions of frankincense was developed. The proposed strategy may serve as a method for the holistic screening of bioactive metabolites in complex TCM samples.

KW - Frankincense resins

KW - Mass spectrometry

KW - Metabolic profiling

KW - Nuclear magnetic resonance

KW - Triterpenoid metabolites

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DO - 10.1016/j.jchromb.2014.05.054

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JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

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Comprehensive identification of active triterpenoid metabolites in frankincense using a coupling strategy

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